Application of the AhR reporter gene assay for the determination of PCDD/Fs and DL-PCBs in feed samples

被引：3

作者：

Piskorska-Pliszczynska, Jadwiga ^{[1
]}

Malagocki, Pawel ^{[1
]}

Furga, Beata ^{[1
]}

Gembal, Magdalena ^{[1
]}

Cebulska, Joanna ^{[1
]}

机构：

[1] Natl Vet Res Inst, Radiobiol Dept, PL-24100 Pulawy, Poland

来源：

JOURNAL OF VETERINARY RESEARCH | 2017年 / 61卷 / 04期

关键词：

feedstuffs; dioxins; dioxin-like polychlorinated biphenyls; bioassay; TOXIC EQUIVALENCY FACTORS; CALUX BIOASSAY; DIOXINS; FOOD; VALIDATION; CHEMICALS; SYSTEMS;

D O I：

10.1515/jvetres-2017-0066

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Introduction: Polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) belong to a well-known group of pollutants. Present in feedstuffs, they bioaccumulate in tissues of food-producing animals. Food is the source of over 90% of human PCDD/Fs and DL-PCBs intake. Thus, feed control is one of the pillars of the EU strategy and a mean of reducing human exposure. The article presents AhR based reporter gene bioassay method for PCDD/Fs and DL-PCBs analysis in feed and its validation results. Material and Methods: Analytes were extracted from samples with fat. Subsequently, fat and other interferences were removed from extract using sulphuric acid modified silica. Extract was further cleaned and PCDD/Fs separated from DL-PCBs using carbon column. Contaminants detection was performed using H1L6.1c3 cell line, which produces luciferase in response to AhR ligands present in extract. Results: Performance characteristics (repeatability, reproducibility, and apparent recovery) fulfil the requirements of Regulation 2017/771/EU. The positive correlation between bioassay and reference HRGC-HRMS method was confirmed. Moreover, the role of screening method used in connection with the confirmatory HRGC-HRMS method in providing feed and food safety has been discussed. Conclusion: Bioassay is a useful method for dioxin and DL-PCBs analysis, allowing cost reduction of monitoring programmes with minimal risk of false negative results.

引用

页码：473 / 481

页数：9

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