A programmable culture platform for hydrostatic stimulation and in situ pH sensing of lung cancer cells with organic electrochemical transistors

被引：8

作者：

Nicol, Cacocciola ^{[1
]}

Parmeggiani, Matteo ^{[1
]}

Villata, Simona ^{[1
,2
]}

Baruffaldi, Desiree ^{[1
,2
]}

Marasso, Simone Luigi ^{[1
,3
]}

Canavese, Giancarlo ^{[1
]}

Cocuzza, Matteo ^{[1
,3
]}

Pirri, Candido Fabrizio ^{[1
,4
]}

Frascella, Francesca ^{[1
,2
]}

机构：

[1] DISAT Politecn Torino, Corso Duca Abruzzi 24, I-10129 Turin, Italy

[2] Politecn Torino, PolitoBIOMed Lab, Cso Duca Abruzzi 24, I-10129 Turin, Italy

[3] CNR IMEM, Parco Area Sci 37a, I-43124 Parma, Italy

[4] Italian Inst Technol, Ctr Sustainable Future Technol, Via Livorno 60, I-10144 Turin, Italy

来源：

MICRO AND NANO ENGINEERING | 2022年 / 16卷

关键词：

Lung-on-a-chip; Bioreactor; Lung cancer; Precision medicine; Organic electrochemical transistors; pH-sensing;

D O I：

10.1016/j.mne.2022.100147

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this work, we present a programmable dynamic cell culture stimulation platform designed to work in a CO2 incubator, compatible with a standard multi-well plate. An epithelial lung cell culture can be mechanically stimulated with a cyclic increase of the hydrostatic pressure inside the culture chamber in the range 1-12 kPa, trying to mimic the human respiration rate. An in situ live pH-monitoring system based on PEDOT:PSS Organic ElectroChemical Transistors was also designed, fabricated and characterized, in order to merge both the stimulated cell culture and the pH sensing in a compact platform.

引用

页数：8

共 30 条

[1] A liver-on-a-chip platform with bioprinted hepatic spheroids [J].

Bhise, Nupura S. ;

Manoharan, Vijayan ;

Massa, Solange ;

Tamayol, Ali ;

Ghaderi, Masoumeh ;

Miscuglio, Mario ;

Lang, Qi ;

Zhang, Yu Shrike ;

Shin, Su Ryon ;

Calzone, Giovanni ;

Annabi, Nasim ;

Shupe, Thomas D. ;

Bishop, Colin E. ;

Atala, Anthony ;

Dokmeci, Mehmet R. ;

Khademhosseini, Ali .

BIOFABRICATION, 2016, 8 (01)

[2] Organic Electrochemical Transistors for the Detection of Cell Surface Glycans [J].

Chen, Lizhen ;

Fu, Ying ;

Wang, Naixiang ;

Yang, Anneng ;

Li, Yuanzhe ;

Wu, Jie ;

Ju, Huangxian ;

Yan, Feng .

ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (22) :18470-18477

[3] Endoscopic sensing of alveolar pH [J].

Choudhury, D. ;

Tanner, M. G. ;

Mcaughtrie, S. ;

Yu, F. ;

Mills, B. ;

Choudhary, T. R. ;

Seth, S. ;

Craven, T. H. ;

Stone, J. M. ;

Mati, I. K. ;

Campbell, C. J. ;

Bradley, M. ;

Williams, C. K. I. ;

Dhaliwal, K. ;

Birks, T. A. ;

Thomson, R. R. .

BIOMEDICAL OPTICS EXPRESS, 2017, 8 (01) :243-259

[4] Dual pO2/pCO2 fibre optic sensing film [J].

Davenport, John J. ;

Hickey, Michelle ;

Phillips, Justin P. ;

Kyriacou, Panayiotis A. .

ANALYST, 2017, 142 (10) :1711-1719

[5] Digestion-on-a-chip: a continuous-flow modular microsystem recreating enzymatic digestion in the gastrointestinal tract [J].

de Haan, Pim ;

Ianovska, Margaryta A. ;

Mathwig, Klaus ;

van Lieshout, Glenn A. A. ;

Triantis, Vassilis ;

Bouwmeester, Hans ;

Verpoorte, Elisabeth .

LAB ON A CHIP, 2019, 19 (09) :1599-1609

[6] Immobilization of enzyme on long period grating fibers for sensitive glucose detection [J].

Deep, Akash ;

Tiwari, Umesh ;

Kumar, Parveen ;

Mishra, Vandana ;

Jain, Subhash C. ;

Singh, Nahar ;

Kapur, Pawan ;

Bharadwaj, Lalit M. .

BIOSENSORS & BIOELECTRONICS, 2012, 33 (01) :190-195

[7] A sensitive optic fiber sensor based on CdSe QDs fluorophore for nitric oxide detection [J].

Ding, Liyun ;

Fan, Chao ;

Zhong, Yunming ;

Li, Tao ;

Huang, Jun .

SENSORS AND ACTUATORS B-CHEMICAL, 2013, 185 :70-76

[8]

Dong L., 2012, CANC PREVENTION FROM, DOI [10.5772/28692, DOI 10.5772/28692]

[9] A two-photon ratiometric fluorescent probe for effective monitoring of lysosomal pH in live cells and cancer tissues [J].

Ge, Jinyin ;

Fan, Li ;

Zhang, Kai ;

Ou, Ting ;

Li, Yinhui ;

Zhang, Caihong ;

Dong, Chuan ;

Shuang, Shaomin ;

Wong, Man Shing .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 262 :913-921

[10] Human Organ Chip Models Recapitulate Orthotopic Lung Cancer Growth, Therapeutic Responses, and Tumor Dormancy In Vitro [J].

Hassell, Bryan A. ;

Goyal, Girija ;

Lee, Esak ;

Sontheimer-Phelps, Alexandra ;

Levy, Oren ;

Chen, Christopher S. ;

Ingber, Donald E. .

CELL REPORTS, 2017, 21 (02) :508-516

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