A guide to standardise artificial contamination procedures with protozoan parasite oocysts or cysts during method evaluation, using Cryptosporidium and leafy greens as models

被引：4

作者：

Chalmers, Rachel M. ^{[1
,2
]}

Katzer, Frank ^{[3
]}

La Carbona, Stephanie ^{[4
]}

Lalle, Marco ^{[5
]}

Razakandrainibe, Romy ^{[6
,7
]}

Robertson, Lucy J. ^{[8
]}

Robinson, Guy ^{[1
,2
]}

Soba, Barbara ^{[9
]}

Temesgen, Tamirat ^{[8
]}

Mayer-Scholl, Anne ^{[10
]}

机构：

[1] Singleton Hosp, Cryptosporidium Reference Unit, Publ Hlth Wales Microbiol & Hlth Protect, Swansea SA2 8QA, W Glam, Wales

[2] Swansea Univ Med Sch, Singleton Pk, Swansea SA2 8QA, W Glam, Wales

[3] Moredun Res Inst, Pentlands Sci Pk, Edinburgh EH26 0PZ, Midlothian, Scotland

[4] ACTALIA Food Safety Dept, 310 Rue Popielujko, F-50000 St Lo, France

[5] Ist Supe Riore Sanita, Unit Foodborne & Neglected Parasit Dis, Dept Infect Dis, Viale Regina Elena 299, I-00161 Rome, Italy

[6] Univ Rouen, EA 7510, ESCAPE, Normandie, France

[7] CHU Rouen, Lab Parasitol Mycol, Ctr Natl Reference Lab Expert Cryptosporidioses, Rouen, France

[8] Norwegian Univ Life Sci, Fac Vet Med, Lab Parasitol, Oluf Thesens Vei 22, As, Norway

[9] Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Zaloska 4, Ljubljana 1000, Slovenia

[10] German Fed Inst Risk Assessment, Dept Biol Safety, Unit Diagnost, Pathogen Characterizat,Parasites Food, Max Dohrn Str 8-10, D-10589 Berlin, Germany

来源：

FOOD CONTROL | 2022年 / 134卷

关键词：

Cryptosporidium; Food; Detection method; Validation; Artificial contamination; GIARDIA CYSTS; CYCLOSPORA-CAYETANENSIS; PARVUM OOCYSTS; TOXOPLASMA-GONDII; FRESH PRODUCE; LETTUCE; VEGETABLES; RECOVERY; TIME; RASPBERRIES;

D O I：

10.1016/j.foodcont.2021.108678

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Protozoan parasites have emerged as a cause of disease associated with fresh produce and berry fruits, and are of particular concern for both public health and the food industry. For example, contamination with Cryptosporidium oocysts, whether directly from faeces or through water used in food production and processing, has led to widespread foodborne outbreaks of cryptosporidiosis. The main foodstuffs implicated so far have been fresh produce (especially leafy greens), fruit juice, milk and dairy products. There is an international standard, ISO 18744:2016, based on microscopy, for the detection of oocysts from leafy green vegetables and berry fruits, but verification and validation data that have been published for this and alternative methods can be difficult to compare due to differences in artificial contamination protocols. There is a lack of reporting of the efficiency and performance of methods used in sample surveys, hampering understanding of parasite occurrence. To improve the consistency and comparability of assays and surveys reporting the results of such artificial contamination experiments, we have developed guidance for artificial contamination procedures and analysis that can be applied to food within the category fresh produce and fruits, with particular attention to leafy greens. Information gathered through an opinion survey revealed that molecular detection would be a valuable development, but that standardised methods and improved validation data were required. A market survey revealed that the provision of oocysts for artificial contamination studies has focused on meeting requirements for microscopy detection. An insight-generation workshop provided the background knowledge synthesised into best practice guidance for artificial contamination studies using either microscopical or molecular detection. This should contribute to better method development and reporting, and improved food safety.

引用

页数：11

共 54 条

[51] The effects of time and temperature on flow cytometry enumerated live Cryptosporidium parvum oocysts [J].