Fractionation, amino acid profiles, antimicrobial and free radical scavenging activities of Citrullus lanatus seed protein

被引:21
作者
Dash, Priyanka [1 ]
Ghosh, Goutam [1 ]
机构
[1] Siksha O Anusandhan Univ, Sch Pharmaceut Sci, Dept Pharmacognosy, Bhubaneswar, Orissa, India
关键词
Citrullus lanatus; prolamin; globulin; antioxidant; antimicrobial; ANTIOXIDANT ACTIVITY; PEPTIDES; ANTIBACTERIAL;
D O I
10.1080/14786419.2017.1305385
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In the present study, a modified Osborne fractionation method was followed to isolate albumin (C-alb), globulin (C-glo), prolamin (C-pro) and glutelin (C-glu) successively from seeds of Citrullus lanatus (watermelon). This research work was undertaken to investigate the antimicrobial and antioxidant activities of isolated protein fractions of C. lanatus seed. Amino acid composition and molecular weight distribution were determined to establish their relationship with antimicrobial and antioxidant activity. Among all the fractions, C-pro was found to be most effective against A. baumannii followed by C-alb and C-glo. The results showed that growth of inhibition of these protein fractions differ significantly from each other (p0.05). In view of antioxidant potential, C-glo exhibited strongest antioxidant capacity while C-glu showed weakest antioxidant potential. [GRAPHICS]
引用
收藏
页码:2945 / 2947
页数:3
相关论文
共 11 条
[1]   Total phenolic content and antioxidant activity of different streams resulting from pilot-plant processes to obtain Amaranthus mantegazzianus protein concentrates [J].
Castel, Virginia ;
Andrich, Oscar ;
Netto, Flavia M. ;
Santiago, Liliana G. ;
Carrara, Carlos R. .
JOURNAL OF FOOD ENGINEERING, 2014, 122 :62-67
[2]   Ocatin.: A novel tuber storage protein from the Andean tuber crop oca with antibacterial and antifungal activities [J].
Flores, T ;
Alape-Girón, A ;
Flores-Díaz, M ;
Flores, HE .
PLANT PHYSIOLOGY, 2002, 128 (04) :1291-1302
[3]   Physicochemical characterisation and radical-scavenging activity of Cucurbitaceae seed oils [J].
Jorge, Neuza ;
da Silva, Ana Carolina ;
Malacrida, Cassia Roberta .
NATURAL PRODUCT RESEARCH, 2015, 29 (24) :2313-2317
[4]   Pyrazole alkaloids from watermelon (Citrullus lanatus) seeds [J].
Kikuchi, Takashi ;
Ikedaya, Aoi ;
Toda, Akiko ;
Ikushima, Kenji ;
Yamakawa, Takahiro ;
Okada, Rina ;
Yamada, Takeshi ;
Tanaka, Reiko .
PHYTOCHEMISTRY LETTERS, 2015, 12 :94-97
[5]   A napin-like polypeptide with translation-inhibitory, trypsin-inhibitory, antiproliferative and antibacterial activities from kale seeds [J].
Ngai, PHK ;
Ng, TB .
JOURNAL OF PEPTIDE RESEARCH, 2004, 64 (05) :202-208
[6]   A new peptide of melon seeds which shows sequence homology with vicilin: Partial characterization and antifungal activity [J].
Ribeiro, S. F. F. ;
Agizzio, A. P. ;
Machado, O. L. T. ;
Neves-Ferreira, A. G. C. ;
Oliveira, M. A. ;
Fernandes, K. V. S. ;
Carvalho, A. O. ;
Perales, Jonas ;
Gomes, V. M. .
SCIENTIA HORTICULTURAE, 2007, 111 (04) :399-405
[7]   Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha) [J].
Sakanaka, S ;
Tachibana, Y ;
Okada, Y .
FOOD CHEMISTRY, 2005, 89 (04) :569-575
[8]   Identification and characterization of antioxidant peptides from chickpea protein hydrolysates [J].
Torres-Fuentes, Cristina ;
del Mar Contreras, Maria ;
Recio, Isidra ;
Alaiz, Manuel ;
Vioque, Javier .
FOOD CHEMISTRY, 2015, 180 :194-202
[9]   Phytochemical screening and HPTLC finger printing analysis of Citrullus lanatus (Thunb.) seed [J].
Varghese, Sumam ;
Narmadha, R. ;
Gomathi, D. ;
Kalaiselvi, M. ;
Devaki, K. .
JOURNAL OF ACUTE DISEASE, 2013, 2 (02) :122-126
[10]   Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate [J].
Xie, Zhengjun ;
Huang, Junrong ;
Xu, Xueming ;
Jin, Zhengyu .
FOOD CHEMISTRY, 2008, 111 (02) :370-376