Environment and virulence factors of Vibrio cholerae strains isolated in Argentina

被引:15
作者
Fraga, S. Gonzalez
Pichel, M.
Costagliola, M.
Cecilia, M.
Jurquiza, V.
Peressutti, S.
Caffer, M. I.
Aulet, O.
Hozbor, C.
Tracanna, B. C.
de Gamundi, A. V.
Hernandez, D.
Ramirez, F. C.
Akselman, R.
Binsztein, N.
机构
[1] Inst Nacl Enfermedades Infecciosas ANLIS Carlos G, Dept Bacteriol, Buenos Aires, DF, Argentina
[2] Inst Nacl Invest & Desarrollo Pesquero, Mar Del Plata, Argentina
[3] Univ Nacl Tucuman, Inst Limnol Noroeste Argentino, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
关键词
antimicrobial resistance; Argentina; environment; plankton; Vibrio cholerae; virulence factors;
D O I
10.1111/j.1365-2672.2007.03468.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To determine the presence of Vibrio cholerae in different areas of Argentina in three sample types, to determine the composition of planktonic communities in areas at which this pathogen was detected and to characterize the virulence properties and antimicrobial resistance of the recovered environmental isolates. Methods and Results: Water and plankton samples were collected in marine, brackish and freshwater environments. Vibrio cholerae non-O1, non-O139 was isolated in 36.1% of the samples analysed. The micro-organism was detected in freshwater but not in marine or brackish samples. No relationship was found between isolation of V. cholerae and presence of any species of plankton. All the isolates presented very similar virulence profiles by PCR, lacking ctxA and tcpA El Tor and containing hlyA (98.7%), rtxA (99.0%), toxR (98.7%) and stn-sto (1.9%). Resistance to ampicillin was found in both Tucuman (21%) and Buenos Aires isolates (45%). Conclusions: We identified two geographic areas in Argentina where V. cholerae was present: freshwaters of the rivers from Tucuman and the Rio de la Plata. Significance and Impact of the Study: The identification of V. cholerae strains in the environment, carrying both virulence factors and resistance to antimicrobial agents, highlight the need for a continuous and active surveillance of this pathogen.
引用
收藏
页码:2448 / 2456
页数:9
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