Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy

被引：12

作者：

Davis, Janel L. ^{[1
]}

Dong, Biqin ^{[1
,2
]}

Sun, Cheng ^{[2
]}

Zhang, Hao F. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA

[2] Northwestern Univ, Dept Mech Engn, Evanston, IL 60208 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2018年 / 23卷 / 10期

关键词：

single-molecule localization microscopy; fluorescence spectral imaging; single-molecule spectroscopy; fluorescent impurities; SUPERRESOLUTION MICROSCOPY; NUCLEIC-ACIDS; DNA ORIGAMI; PROTEINS; PROBES; SPECTROSCOPY; PHOTOPHYSICS; STORM; FISH;

D O I：

10.1117/1.JBO.23.10.106501

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The existence of fluorescent impurities has been a long-standing obstacle in single-molecule imaging, which results in sample misidentification and higher localization uncertainty. Spectroscopic single-molecule localization microscopy can record the full fluorescent spectrum of every stochastic single-molecule emission event. This capability allows us to quantify the spatial and spectral characteristics of fluorescent impurities introduced by sample preparation steps, based on which we developed a method to effectively separate fluorescent impurities from target molecules. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.

引用

页数：14

共 38 条

[1] Reduction of luminescent background in ultrasensitive fluorescence detection by photobleaching [J].

Affleck, RL ;

Ambrose, WP ;

Demas, JN ;

Goodwin, PM ;

Schecker, JA ;

Wu, JM ;

Keller, RA .

ANALYTICAL CHEMISTRY, 1996, 68 (13) :2270-2276

[2]

[Anonymous], 2015, Nature, V527, P544, DOI 10.1038/nature15745

[3] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].

Bates, Mark ;

Huang, Bo ;

Dempsey, Graham T. ;

Zhuang, Xiaowei .

SCIENCE, 2007, 317 (5845) :1749-1753

[4] Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes [J].

Bates, Mark ;

Huang, Bo ;

Zhuang, Xiaowei .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2008, 12 (05) :505-514

[5] Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes [J].

Beliveau, Brian J. ;

Boettiger, Alistair N. ;

Avendano, Maier S. ;

Jungmann, Ralf ;

McCole, Ruth B. ;

Joyce, Eric F. ;

Kim-Kiselak, Caroline ;

Bantignies, Frederic ;

Fonseka, Chamith Y. ;

Erceg, Jelena ;

Hannan, Mohammed A. ;

Hoang, Hien G. ;

Colognori, David ;

Lee, Jeannie T. ;

Shih, William M. ;

Yin, Peng ;

Zhuang, Xiaowei ;

Wu, Chao-ting .

NATURE COMMUNICATIONS, 2015, 6

[6] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[7] Spatially resolved, highly multiplexed RNA profiling in single cells [J].

Chen, Kok Hao ;

Boettiger, Alistair N. ;

Moffitt, Jeffrey R. ;

Wang, Siyuan ;

Zhuang, Xiaowei .

SCIENCE, 2015, 348 (6233)

[8] Comparison of chemical cleaning methods of glass in preparation for silanization [J].

Cras, JJ ;

Rowe-Taitt, CA ;

Nivens, DA ;

Ligler, FS .

BIOSENSORS & BIOELECTRONICS, 1999, 14 (8-9) :683-688

[9]

Culley S, 2018, NAT METHODS, V15, P263, DOI [10.1038/NMETH.4605, 10.1038/nmeth.4605]

[10] Stochastic fluorescence switching of nucleic acids under visible light illumination [J].

Dong, Biqin ;

Almassalha, Luay M. ;

Soetikno, Brian T. ;

Chandler, John E. ;

The-Quyen Nguyen ;

Urban, Ben E. ;

Sun, Cheng ;

Zhang, Hao F. ;

Backman, Vadim .

OPTICS EXPRESS, 2017, 25 (07) :7929-7944

← 1 2 3 4 →