Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection

被引：44

作者：

Du, Taofeng ^{[1
,2
]}

Shi, Yunpeng ^{[1
,2
]}

Xiao, Shuqi ^{[1
,2
]}

Li, Na ^{[1
,2
]}

Zhao, Qin ^{[1
,2
]}

Zhang, Angke ^{[1
,2
]}

Nan, Yuchen ^{[1
,2
]}

Mu, Yang ^{[1
,2
]}

Sun, Yani ^{[1
,2
]}

Wu, Chunyan ^{[1
,2
]}

Zhang, Hongtao ^{[3
]}

Zhou, En-Min ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, Dept Prevent Vet Med, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

[2] Minist Agr, Expt Stn Vet Pharmacol & Vet Biotechnol, Yangling 712100, Shaanxi, Peoples R China

[3] Univ Penn, Dept Pathol & Lab Med, Perelman Sch Med, 3620 Hamilton Walk, Philadelphia, PA 19104 USA

来源：

BMC VETERINARY RESEARCH | 2017年 / 13卷

基金：

中国国家自然科学基金;

关键词：

PRRSV; Curcumin; Binding; Internalization; Virus-mediated cell fusion; IN-VITRO; ALVEOLAR MACROPHAGES; REPLICATION; SWINE;

D O I：

10.1186/s12917-017-1218-x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) could lead to pandemic diseases and huge financial losses to the swine industry worldwide. Curcumin, a natural compound, has been reported to serve as an entry inhibitor of hepatitis C virus, chikungunya virus and vesicular stomatitis virus. In this study, we investigated the potential effect of curcumin on early stages of PRRSV infection. Results: Curcumin inhibited infection of Marc-145 cells and porcine alveolar macrophages (PAMs) by four different genotype 2 PRRSV strains, but had no effect on the levels of major PRRSV receptor proteins on Marc-145 cells and PAMs or on PRRSV binding to Marc-145 cells. However, curcumin did block two steps of the PRRSV infection process: virus internalization and virus-mediated cell fusion. Conclusions: Our results suggested that an inhibition of genotype 2 PRRSV infection by curcumin is virus strain-independent, and mainly inhibited by virus internalization and cell fusion mediated by virus. Collectively, these results demonstrate that curcumin holds promise as a new anti-PRRSV drug.

引用

页数：9

共 28 条

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