pH dependent uptake of porphyrin-type photo sensitizers by solid tumor cells in vitro is not induced by modification of transmembrane potential

被引：15

作者：

Cunderlíkova, B

Moan, J

Sjaastad, I

机构：

[1] Int Laser Ctr, Bratislava 81219, Slovakia

[2] Canc Res Inst, Dept Biophys, N-0310 Oslo, Norway

[3] Univ Oslo, Dept Phys, N-0316 Oslo, Norway

[4] Ullevaal Univ Hosp, Expt Med Res Inst, N-0407 Oslo, Norway

[5] Ullevaal Univ Hosp, Heart & Lung Ctr, Dept Cardiol, N-0407 Oslo, Norway

来源：

CANCER LETTERS | 2005年 / 222卷 / 01期

关键词：

photodynamic therapy; pH-dependence; transmembrane potential; cellular uptake;

D O I：

10.1016/j.canlet.2004.09.032

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The uptake of HpIX, TPPS2a and mTHPC by WiDr, THX cells and skin fibroblasts at pH 7.4 and 6.8 was compared. In the absence of serum, the uptake of HpIX was higher at lower pH. The difference was significant in WiDr cells (P < 0.01) and skill fibroblasts (P < 0.05). TPPS2a nor mTHPC showed any pH dependent uptake. Lowering the extracellular pH resulted in a significant depolarization (3-8 mV) of the cells. Application of tetraethylammonium chloride did not affect the cellular uptake of any of the photosensitizers. We conclude that the pH dependent Uptake of photosensitizers is not mainly related to altered transmembrane potential. (c) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：39 / 47

页数：9

共 44 条

[1] PHOTODYNAMIC THERAPY OF CHEMICALLY-INDUCED AND ULTRAVIOLET-B RADIATION-INDUCED MURINE SKIN PAPILLOMAS BY CHLOROALUMINUM PHTHALOCYANINE TETRASULFONATE [J].