Isolation of two plant proteinases in latex from Carica candamarcensis acting as mitogens for mammalian cells

被引:26
作者
Gomes, MTR
Mello, VJ
Rodrigues, KC
Bemquerer, MP
Lopes, MTR
Faça, VM
Salas, CE
机构
[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Farmacol, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Imunol, BR-31270901 Belo Horizonte, MG, Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Quim Prot, Ribeirao Preto, Brazil
关键词
Carica candamarcensis; cariaceae; latex; mitogens; proteinases;
D O I
10.1055/s-2005-837824
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
in a prior study we showed evidence that latex from Carica candamarcensis contains a protein fraction that stimulates mammalian-cell proliferation. In this report we describe the isolation of two proteinases responsible for this effect. Both proteinases (P1, P2) display a relative mass of 23 kDa and following chromatographic purification stimulate proliferation of fibroblastic and epithelial cells. P2 added to L929 fibroblasts at 2.5 nM enhances proliferation by 60%. We further demonstrate that its cellular effect is linked to an increase in activity of Erk2, a component of the MAP kinase pathway. To our knowledge, this is the first known plant proteinase to exert a proliferative effect in mammalian cells. This novel mitogenic property attributed to a purified cysteine proteinase may explain some of the therapeutic actions attributed to these enzymes.
引用
收藏
页码:244 / 248
页数:5
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