Inhibitory effect of MIL glycoprotein on expression of pro-inflammatory mediators in carbon tetrachloride-induced mice liver damage

被引：3

作者：

Oh, Phil-Sun ^{[1
,2
]}

Lee, Jin ^{[1
,2
]}

Lim, Kye-Taek ^{[1
]}

机构：

[1] Chonnam Natl Univ, Biotechnol Res Inst, Mol Biochem Lab, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Ctr Control Anim Hazards Using Biotechnol BK21, Kwangju 500757, South Korea

来源：

JOURNAL OF APPLIED TOXICOLOGY | 2010年 / 30卷 / 08期

关键词：

Morus Indica Linne (MIL) glycoprotein; liver; COX-2; TNF-alpha; IL-1; beta; NF-KAPPA-B; SIGNAL-TRANSDUCTION PATHWAYS; PROTEIN-KINASE MAPK; C-JUN; RAT-LIVER; TERMINAL KINASE; REACTIVE OXYGEN; CELL-DEATH; ACTIVATION; HEPATOTOXICITY;

D O I：

10.1002/jat.1557

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The aim of the present study was to evaluate immunomodulatory and hepatoprotective effects of glycoprotein isolated from Morus indica Linne (MIL glycoprotein) on carbon tetrachloride (CCl4)-induced liver injury. In the present study, MIL glycoprotein (5 and 10 mg kg(-1) body weight) was administered to ICR mice for 7 days prior to CCl4 treatment. We evaluated the activities of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and thiobarbituric acid-reactive substances (TBARS), and expression of inflammation-related mediators including cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta in CCl4-treated mice. Our results revealed that MIL glycoprotein reduced the activities of ALT, LDH and TBARS in serum from CCl4-treated mice. We also found that MIL glycoprotein reduced the activity of COX-2 and expression of TNF-alpha and 1L-1 beta in liver from CCl4-treated mice. Moreover, administration of MIL glycoprotein suppressed on stress-activated protein kinase/c-jun N-terminal kinase phosphorylation and activator protein-1 transcriptional activation in livers from CCl4-treated mice. The results from these experiments indicate that MIL glycoprotein effectively protects against liver injury, mainly through downregulation of oxidative stress and the inflammatory response. In conclusion, we suggest that the MIL glycoprotein might be one component of health supplements for prevention of liver diseases. Copyright (C) 2010 John Wiley & Sons, Ltd.

引用

页码：754 / 760

页数：7

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