β-Carotene down-regulates inducible nitric oxide synthase gene expression and induces apoptosis by suppressing bcl-2 expression and activating caspase-3 and p53 genes in B16F-10 melanoma cells

被引：18

作者：

Guruvayoorappan, Chandrasekharan ^{[1
]}

Kuttan, Girija ^{[1
]}

机构：

[1] Amala Canc Res Ctr, Dept Immunol, Trichur 680555, Kerala, India

来源：

NUTRITION RESEARCH | 2007年 / 27卷 / 06期

关键词：

apoptosis; beta-carotene; bcl-2; caspase-3; iNOS; B16F-10 melanoma cells;

D O I：

10.1016/j.nutres.2007.04.009

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The objective of this research was to assess the effect of fl-carotene on the regulation of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha production and stimulation of apoptosis in B1617-10 melanoma cells. P-Carotene at a concentration of 10 mu g/mL could significantly (P <.01) inhibit NO and TNF-oc production in B1617-10 melanoma cells. This was further evidenced by the regulatory effect of beta-carotene on the inhibition of inducible nitric oxide synthase gene expression in B16F-10 cells. We investigated the effect of fl-carotene on induction of apoptosis in B16F-10 cells. Incubation of B16F-10 cells with fl-carotene showed the presence of apoptotic bodies and induced DNA fragmentation. Furthermore, fl-carotene showed inhibitory effect on bcl-2 expression and upregulated p53 and caspase-3 gene expression in B16F-10 melanoma cells. In conclusion, the observed results suggest that beta-carotene could regulate NO and TNF-alpha production and stimulates apoptosis in B16F-10 melanoma cells by regulating bcl-2, p53, and caspase-3 genes. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：336 / 342

页数：7

共 46 条

[31] β-Carotene:: a cancer chemopreventive agent or a co-carcinogen? [J].

Paolini, M ;

Abdel-Rahman, SZ ;

Sapone, A ;

Pedulli, GF ;

Perocco, P ;

Cantelli-Forti, G ;

Legator, MS .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2003, 543 (03) :195-200

[32] Effect of β-Carotene on the inhibition of lung metastasis in mice [J].

Pradeep, CR ;

Kuttan, G .

PHYTOMEDICINE, 2003, 10 (2-3) :159-164

[33] Apoptosis induction in renal cell carcinoma by TRAIL and γ-radiation is impaired by deficient caspase-9 cleavage [J].

Ramp, U ;

Caliskan, E ;

Mahotka, C ;

Krieg, A ;

Heikaus, S ;

Gabbert, HE ;

Gerharz, CD .

BRITISH JOURNAL OF CANCER, 2003, 88 (11) :1800-1807

[34] p53 regulates the minimal promoter of the human topoisomerase II alpha gene [J].

Sandri, MI ;

Isaacs, RJ ;

Ongkeko, WM ;

Harris, AL ;

Hickson, ID ;

Broggini, M ;

Vikhanskaya, F .

NUCLEIC ACIDS RESEARCH, 1996, 24 (22) :4464-4470

[35] The role of NF-κB/IκB proteins in cancer:: implications for novel treatment strategies [J].

Schwartz, SA ;

Hernandez, A ;

Evers, BM .

SURGICAL ONCOLOGY-OXFORD, 1999, 8 (03) :143-153

[36]

Suganuma M, 1996, CANCER RES, V56, P3711

[37] RECOMBINANT HUMAN-TUMOR NECROSIS FACTOR-ALPHA - EFFECTS ON PROLIFERATION OF NORMAL AND TRANSFORMED-CELLS INVITRO [J].

SUGARMAN, BJ ;

AGGARWAL, BB ;

HASS, PE ;

FIGARI, IS ;

PALLADINO, MA ;

SHEPARD, HM .

SCIENCE, 1985, 230 (4728) :943-945

[38]

Tanaka H, 1999, INT J ONCOL, V14, P1069

[39]

THOMSEN LL, 1994, CANCER RES, V54, P1352

[40] THE L929-CELL BIOASSAY FOR MURINE TUMOR-NECROSIS-FACTOR IS NOT INFLUENCED BY OTHER MURINE CYTOKINES [J].

TOMKINS, P ;

COOPER, K ;

WEBBER, D ;

BOWEN, G .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 151 (1-2) :313-315

← 1 2 3 4 5 →