Use of the multiparametric panel CD3/CD4/CD8/CD7/CD26/CD158k in the detection and use of flow cytometry of Sezary cells

被引：0
作者：
Callet, Jonas [1 ,2 ]
Latger-Cannard, Veronique [1 ,2 ]
Gerard, Delphine [1 ,2 ]
Salignac, Sylvain [1 ,2 ]
Granel-Brocard, Florence [3 ]
Campidelli, Arnaud [4 ]
Bursztejn, Anne-Claire [3 ]
Broseus, Julien [1 ,2 ]
Vial, Jean-Philippe [5 ]
Lesesve, Jean-Francois [1 ,2 ]
机构：
[1] CHRU Nancy, Serv Hematol Biol, Nancy, France
[2] CHRU Nancy, Plateforme Cytometrie Flux, Nancy, France
[3] CHRU Nancy, Serv Dermatol, Nancy, France
[4] CHRU Nancy, Serv Hematol Clin, Nancy, France
[5] CHU Bordeaux, Lab Hematol, Bordeaux, France
来源：
ANNALES DE BIOLOGIE CLINIQUE | 2021年 / 79卷 / 03期
关键词：
multiparametric panel; flow cytometry; sezary; Sezary cells; MYCOSIS-FUNGOIDES; CLASSIFICATION; CD158K/KIR3DL2; DIAGNOSIS; MARKER; SKIN;
D O I：
10.1684/abc.2021.1651
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
The Sezary syndrome has been defined by a triad combining erythroderinia, generalized lymphadenopathy, and the presence of circulating Sezary cells > 1 x 10(9)/L characterized by a CD4+/CD8- phenotype with loss of one or more T antigens (mainly CD7 and/or CD26). We retrospectively reviewed the inununophenotypic profiles of 10 SS patients followed in our institution (University Hospital at Nancy, France). The application of the WHO criteria resulted in a diagnostic confirmation for 9 out of 10 cases. Since 2008, new diagnostic and staging criteria have been proposed. including the CD158k/KIR3DL2 receptor detection. The application of these new criteria to our cohort led us to notice a phenotypic heterogeneity of our cases but allowed to achieve a relevant diagnosis of Sezary syndrome in all cases, especially for patients with lymphopenia. The use of such a panel of monoclonal antibodies also optimized the follow-up of the patients.
引用
收藏
页码：233 / 240
页数：8
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