Optimization of medium constituents and fermentation conditions for the production of L-glutamic acid by the coimmobilized whole cells of Micrococcus glutamicus and Pseudomonas reptilivora

Statistical experimental design was used to optimize medium constituents and the conditions of fermentation, viz., temperature, pH and the time of fermentation. Higher yields of L-glutamic acid (37.1 kg/m(3)) was obtained after optimizing medium components and the conditions of fermentation. The optimal levels of medium components were: 61.5575 kg/m(3) glucose, 7.3272 kg/m(3) urea and 1.783 mu g/dm(3) biotin. The optimum productivity was achieved using optimized medium at the fermentation temperature of 33.7 degrees C, initial pH 7.74, and at the time of fermentation of 58.4 h.