Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila

被引：101
作者：
Blanchette, M
Green, RE
Brenner, SE
Rio, DC
机构：
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Ctr Integrat Genom, Berkeley, CA 94720 USA
来源：
GENES & DEVELOPMENT | 2005年 / 19卷 / 11期
关键词：
alternative splicing; SR proteins; hnRNP proteins; microarray; Drosophila melanogaster;
D O I：
10.1101/gad.1314205
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects > 300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.
引用
收藏
页码：1306 / 1314
页数：9
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