Inhibition of the gravitropic response of snapdragon spikes by the calcium-channel blocker lanthanum chloride

被引:54
作者
Friedman, H
Meir, S
Rosenberger, I
Halevy, AH
Kaufman, PB
Philosoph-Hadas, S [1 ]
机构
[1] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-50250 Bet Dagan, Israel
[2] Hebrew Univ Jerusalem, Fac Agr, Kennedy Leigh Ctr Hort Res, IL-76100 Rehovot, Israel
[3] Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA
关键词
D O I
10.1104/pp.118.2.483
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The putative Ca2+-channel blocker LaCl3 prevented the gravitropic bending of cut snapdragon (Antirrhinum majus L.) spikes (S. Philosoph-Hadas, S. Meir, I. Rosenberger, A.H. Halevy [1996] Plant Physiol 110: 301-310) and inhibited stem curvature to a greater extent than vertical and horizontal stem elongation at the bending zone. This might indicate that LaCl3, which modulates cytosolic Ca2+, does not influence general stem-growth processes but may specifically affect other gravity-associated processes occurring at the stem-bending zone. Two such specific gravity-dependent events were found to occur in the bending zone of snapdragon spikes: sedimentation of starch-containing chloroplasts at the bottom of stem cortex cells, as seen in cross-sections, and establishment of an ethylene gradient across the stem. Our results show that the lateral sedimentation of chloroplasts associated with gravity sensing was prevented in cross-sections taken from the bending zone of LaCl3-treated and subsequently gravistimulated spikes and that LaCl3 completely prevented the gravity-induced, asymmetric ethylene production established across the stem-bending zone. These data indicate that LaCl3 inhibits stem curvature of snapdragon spikes by preventing several gravity-dependent processes. Therefore, we propose that the gravitropic response of shoots could be mediated through a Ca2+-dependent pathway involving modulation of cytosolic Ca2+ at various stages.
引用
收藏
页码:483 / 492
页数:10
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