Construction and characterization of a human bacterial artificial chromosome library

被引：319

作者：

Kim, UJ ^{[1
]}

Birren, BW ^{[1
]}

Slepak, T ^{[1
]}

Mancino, V ^{[1
]}

Boysen, C ^{[1
]}

Kang, HL ^{[1
]}

Simon, MI ^{[1
]}

Shizuya, H ^{[1
]}

机构：

[1] CALTECH,DIV BIOL,PASADENA,CA 91125

来源：

GENOMICS | 1996年 / 34卷 / 02期

关键词：

D O I：

10.1006/geno.1996.0268

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have constructed an arrayed human genomic BAC library with approximately 4x coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 kb. A new BAC vector that allows color-based positive screening to identify transformants with inserts has increased BAC cloning efficiency. The library was gridded onto hybridization filters at high density for efficient identification of BAC clones by colony hybridization. The library was also formulated into characteristic DNA pools to allow for PCR screening of the library for STS content. We have characterized the library mainly by screening with more than 300 different landmarks that include cDNA, STSs, and cosmid clones. We describe methods for using BAC clones and discuss the implications for genome characterization, mapping, and sequencing. (C) 1996 Academic Press, Inc.

引用

页码：213 / 218

页数：6

共 25 条

[21] TRANSFORMATION OF ESCHERICHIA-COLI WITH LARGE DNA-MOLECULES BY ELECTROPORATION [J].