Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

被引：45

作者：

Joo, Jeong Chan ^{[1
]}

Oh, Young Hoon ^{[1
]}

Yu, Ju Hyun ^{[1
]}

Hyun, Sung Min ^{[1
,2
]}

Khang, Tae Uk ^{[1
,3
]}

Kang, Kyoung Hee ^{[1
]}

Song, Bong Keun ^{[1
]}

Park, Kyungmoon ^{[2
]}

Oh, Min-Kyu ^{[3
]}

Lee, Sang Yup ^{[4
]}

Park, Si Jae ^{[5
]}

机构：

[1] Korea Res Inst Chem Technol, Ctr Biobased Chem, Div Convergence Chem, POB 107,141 Gajeong Ro, Daejeon 34602, South Korea

[2] Hongik Univ, Dept Biol & Chem Engn, 2639 Sejong Ro, Sejong Si 30016, South Korea

[3] Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea

[4] Korea Adv Inst Sci & Technol, Metab Engn Natl Res Lab, Dept Chem & Biomol Engn, Plus Program BK21, 291 Daehak Ro, Daejeon 34141, South Korea

[5] Ewha Womans Univ, Div Chem Engn & Mat Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

基金：

新加坡国家研究基金会;

关键词：

Corynebacterium glutamicum; Metabolic engineering; 5-Aminovaleric acid; Miscanthus; Pretreatment; SUBSEQUENT ENZYMATIC-HYDROLYSIS; HIGH-LEVEL CONVERSION; ESCHERICHIA-COLI; L-LYSINE; CHEMICALS; BIOMASS; HYDROTHERMOLYSIS; EXPRESSION; CADAVERINE; GLUTARATE;

D O I：

10.1016/j.biortech.2017.05.131

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93 g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51 g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：1692 / 1700

页数：9

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