Freeform direct laser writing of versatile topological 3D scaffolds enabled by intrinsic support hydrogel

被引：11

作者：

Hasselmann, Sebastian ^{[1
]}

Hahn, Lukas ^{[2
]}

Lorson, Thomas ^{[2
]}

Schaetzlein, Eva ^{[3
]}

Sebastien, Isabelle ^{[4
]}

Beudert, Matthias ^{[5
]}

Luehmann, Tessa ^{[5
]}

Neubauer, Julia C. ^{[4
]}

Sextl, Gerhard ^{[6
]}

Luxenhofer, Robert ^{[2
,7
,8
]}

Heinrich, Doris ^{[6
,9
,10
]}

机构：

[1] Fraunhofer Project Ctr Stem Cell Proc Engn, Neunerpl 2, D-97082 Wiirzburg, Germany

[2] Univ Wurzburg, Dept Chem & Pharm, Inst Funct Mat & Biofabricat, Funct Polymer Mat,Chair Adv Mat Synth, Rontgenring 11, D-97070 Wurzburg, Germany

[3] East Bavarian Tech Univ Appl Sci, Prufrninger Str 58, D-93049 Regensburg, Germany

[4] Fraunhofer Project Ctr Stem Cell Proc Engn, Fraunhofer Inst Biomed Engn, Neunerpl 2, D-97082 Wurzburg, Germany

[5] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany

[6] Fraunhoftr Inst Silicate Res ISC, Neunerpl 2, D-97082 Wurzburg, Germany

[7] Univ Helsinki, Soft Matter Chem, Dept Chem, Fac Sci, FIN-00014 Helsinki, Finland

[8] Univ Helsinki, Helsinki Inst Sustainabil Sci, Fac Sci, FIN-00014 Helsinki, Finland

[9] Inst Bioproc & Analyt Measurement Tech, D-37308 Rosenhof, Heilbad Heilige, Germany

[10] Ilmenau Univ Technol, Fac Math & Nat Sci, Ilmenau, Germany

来源：

MATERIALS HORIZONS | 2021年 / 8卷 / 12期

关键词：

CELL-MIGRATION; 2-PHOTON POLYMERIZATION; FABRICATION; ADHESION; DIFFERENTIATION; FIBROBLASTS; TECHNOLOGY; DELIVERY; FORCES;

D O I：

10.1039/d1mh00925g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, a novel approach to create arbitrarily shaped 3D hydrogel objects is presented, wherein freeform two-photon polymerization (2PP) is enabled by the combination of a photosensitive hydrogel and an intrinsic support matrix. This way, topologies without physical contact such as a highly porous 3D network of concatenated rings were realized, which are impossible to manufacture with most current 3D printing technologies. Micro-Raman and nanoindentation measurements show the possibility to control water uptake and hence tailor the Young's modulus of the structures via the light dosage, proving the versatility of the concept regarding many scaffold characteristics that makes it well suited for cell specific cell culture as demonstrated by cultivation of human induced pluripotent stem cell derived cardiomyocytes.

引用

页码：3334 / 3344

页数：11

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