Proteomic Analysis of Wort with Focus on Potential Differentiation of Malting Barley Cultivars

被引：0

作者：

Dusek, Martin ^{[1
]}

Scigelova, Michaela ^{[2
]}

Jandovska, Vladimira ^{[3
]}

Olsovska, Jana ^{[1
]}

机构：

[1] Res Inst Brewing & Malting, Lipova 511-15, Prague 12000, Czech Republic

[2] Thermo Fisher Sci, Hanna Kunath Str 11, D-28199 Bremen, Germany

[3] Charles Univ Prague, Fac Sci, Albertov 6, Prague 12800, Czech Republic

来源：

KVASNY PRUMYSL | 2019年 / 65卷 / 06期

关键词：

barley; malt; cultivars differentiation; proteomics; LC-MS;

D O I：

10.18832/kp2019.65.186

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We analyzed the profile of malt proteins in boiled wort preparations using liquid chromatography coupled to high resolution mass spectrometry. Proteins detected in 9 different malt barley cultivars were identified. We probed proteins for post-translational modifications, namely glycation. Differential analysis of the wort proteomes provided insight into the effect of malting barley cultivar as well as location of cultivation. The results suggest that proteome analysis of wort samples could allow for identification/classification of malting barley cultivars used in wort production.

引用

页码：186 / 191

页数：6

共 9 条

[1]

AMES JM, 1988, CHEM IND-LONDON, P558

[2] Influence of the Brewing Process on the Barley Protein Profile [J].

Benkovska, Dagmar ;

Flodrova, Dana ;

Psota, Vratislav ;

Bobalova, Janette .

KVASNY PRUMYSL, 2011, 57 (7-8) :260-265

[3]

Chmelík J, 2002, ROST VYROBA, V48, P261

[4]

Cordova K., COMMUNICATION

[5]

*EBC AN COMM, 1998, AN EBC

[6] Optimal tocopherol concentrations to inhibit soybean oil oxidation [J].

Evans, JC ;

Kodali, DR ;

Addis, PB .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2002, 79 (01) :47-51

[7]

Fix G., 1999, PRINCIPLES OF BREWIN

[8] 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing [J].

Petry-Podgorska, Inga ;

Zidkova, Jitka ;

Flodrova, Dana ;

Bobalova, Janette .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (30) :3143-3148

[9] MALDI-TOF mass spectrometry of hordeins: rapid approach for identification of malting barley varieties [J].

Salplachta, Jiri ;

Bobalova, Janette .

JOURNAL OF MASS SPECTROMETRY, 2009, 44 (09) :1287-1292

← 1 →