Human glioma cells-conditioned medium promotes proliferation and migration of human bone marrow mesenchymal stromal cells in vitro

Purpose: To investigate changes in cell proliferation and migration of human bone marrow-derived mesenchymal stromal cells (hMSCs) in glioma environment, in order to assess the tumorigenic risk of hMSCs in the clinical application to treat human gliomas. Methods: hMSCs were obtained from normal adult persons and identified by their morphological characteristics and test of their stemness. The U251 glioma cell-conditioned medium (U251-CM) was obtained to simulate the human glioma environment in vitro. hMSCs were cultured in the U251-CM or the control medium in the same conditionzs and changes in cell proliferation and migration were detected by MTT assay and wound healing assay. Results: The results of MTT assay showed that, compared with the control group, the proliferation of hMSCs cultured in U251-CM increased significantly, and the results of wound healing assay showed that the migration of hMSCs cultured in U251-CM also increased significantly. Conclusions: Human glioma cell-conditioned medium may promote the proliferation and migration of hMSCs, and we are concerned about the tumorigenic risk of hMSCs in glioma environment before their clinical application.