Important Role for the Murid Herpesvirus 4 Ribonucleotide Reductase Large Subunit in Host Colonization via the Respiratory Tract

被引：16

作者：

Gill, Michael B. ^{[1
]}

May, Janet S. ^{[1
]}

Colaco, Susanna ^{[1
]}

Stevenson, Philip G. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Pathol, Div Virol, Cambridge CB2 1QP, England

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 20期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

VIRAL GENE-EXPRESSION; IN-VIVO; KAPOSIS-SARCOMA; GAMMAHERPESVIRUS-68; REPLICATION; INFECTION; LATENCY; CELLS; MICE; IDENTIFICATION;

D O I：

10.1128/JVI.00828-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viral enzymes that process small molecules provide potential chemotherapeutic targets. A key constraint-the replicative potential of spontaneous enzyme mutants-has been hard to define with human gammaher-pesviruses because of their narrow species tropisms. Here, we disrupted the murid herpesvirus 4 (MuHV-4) ORF61, which encodes its ribonucleotide reductase (RNR) large subunit. Mutant viruses showed delayed in vitro lytic replication, failed to establish infection via the upper respiratory tract, and replicated to only a very limited extent in the lower respiratory tract without reaching lymphoid tissue. RNR could therefore provide a good target for gammaherpesvirus chemotherapy.

引用

页码：10937 / 10942

页数：6

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