Effects of aloe emodin on U87MG glioblastoma cell growth: In vitro and in vivo study

被引：30

作者：

Arcella, Antonietta ^{[1
]}

Oliva, Maria Antonietta ^{[1
]}

Staffieri, Sabrina ^{[1
]}

Sanchez, Massimo ^{[2
]}

Madonna, Michele ^{[1
]}

Riozzi, Barbara ^{[1
]}

Esposito, Vincenzo ^{[1
,3
]}

Giangaspero, Felice ^{[1
]}

Frati, Luigi ^{[1
]}

机构：

[1] IRCCS NEUROMED, Pozzilli, Italy

[2] Ist Super Sanita, Rome, Italy

[3] Univ Rome Sapienza, Rome, Italy

来源：

ENVIRONMENTAL TOXICOLOGY | 2018年 / 33卷 / 11期

关键词：

adjuvant chemotherapy; aloe emodin; anthraquinone; apoptosis; brain cancer; cell cycle; glioblastoma; natural drug; temozolomide; METABOTROPIC GLUTAMATE RECEPTORS; DIFFERENTIATION; SURVIVAL; GLIOMAS;

D O I：

10.1002/tox.22622

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence other approaches have been investigated to target more pathways involved in glioblastoma development and progression. Here we investigate the anticancer effect of Aloe-Emodin (AE), an anthraquinone compound presents in the leaves of Aloe arborescens, on human glioblastoma cell line U87MG. U87MG were treated with various concentrations of AE (20 and 40M) for different times (24, 48, and 72hr). Cell growth was monitored by daily cell count after treatments. Growth analysis showed that AE significantly decrease proliferation of U87MG in a time and dose dependent manner. FACS analysis demonstrates a block of cell cycle in S and G2/M phase. AE probably induced also apoptosis by releasing of apoptosis-inducing factor: PARP and Lamin activation leading to nuclear shrinkage. In addition, exposure of U87MG to AE reduced pAKT phosphorylation. AE inhibition of U87MG growth is a result of more mechanism together. Here we report that AE has a specific growth inhibition on U87MG also in in vivo. The growth of U87MG, subcutaneously injected in nude mice with severe combined immunodeficiency, is inhibited without any appreciable toxic effects on the animals after AE treatment. AE might represent a conceptually new lead antitumor adjuvant drug.

引用

页码：1160 / 1167

页数：8

共 21 条

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