Small-molecule fulvic acid with strong hydration ability for non-vitreous cellular cryopreservation

被引：7

作者：

Bai, Guoying ^{[1
,3
]}

Hu, Jinhao ^{[1
]}

Qin, Sijia ^{[1
]}

Qi, Zipeng ^{[1
]}

Zhuang, Hening ^{[1
]}

Sun, Fude ^{[2
]}

Lu, Youhua ^{[3
]}

Jin, Shenglin ^{[3
]}

Gao, Dong ^{[2
]}

Wang, Jianjun ^{[3
]}

机构：

[1] Hebei Univ Technol, Sch Mat Sci & Engn, Tianjin 300401, Peoples R China

[2] Hebei Univ Technol, Key Lab Hebei Prov Mol Biophys, Inst Biophys, Tianjin 300401, Peoples R China

[3] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China

来源：

ISCIENCE | 2022年 / 25卷 / 06期

基金：

中国国家自然科学基金;

关键词：

ICE RECRYSTALLIZATION; ANTIFREEZE-PROTEIN; INHIBITION; GROWTH; WATER; GLYCOPROTEINS; RELAXATION; ADSORPTION; CRYSTALS; DYNAMICS;

D O I：

10.1016/j.isci.2022.104423

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The exploitation of biocompatible ice-control materials especially the small molecules for non-vitreous cryopreservation remains challenging. Here, we report a small molecule of fulvic acid (FA) with strong hydration ability, which enables non-vitreous cellular cryopreservation by reducing ice growth during freezing and reducing ice recrystallization/promoting ice melting during thawing. Without adding any other cryoprotectants, FA can enhance the recovery of sheep red blood cells (RBCs) by three times as compared with a commercial cryoprotectant (hydroxyethyl starch) under a stringent test condition. Investigation of water mobility reveals that the ice-control properties of FA can be ascribed to its strong bondage to water molecules. Furthermore, we found that FA can be absorbed by RBCs and mainly locates on membranes, suggesting the possible contribution of FA to cell protection through stabilizing membranes. This work bespeaks a bright future for small-molecule cryoprotectants in non-vitreous cryopreservation application.

引用

页数：17

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