Mass Spectrometry Applied to Bottom-Up Proteomics: Entering the High-Throughput Era for Hypothesis Testing

被引:232
作者
Gillet, Ludovic C. [1 ]
Leitner, Alexander [1 ]
Aebersold, Ruedi [1 ,2 ]
机构
[1] Swiss Fed Inst Technol, Inst Mol Syst Biol, Dept Biol, CH-8093 Zurich, Switzerland
[2] Univ Zurich, Fac Sci, CH-8057 Zurich, Switzerland
来源
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 9 | 2016年 / 9卷
关键词
data-dependent acquisition; data-independent acquisition; peptide-centric; shotgun proteomics; spectrum-centric; targeted proteomics; DATA-INDEPENDENT-ACQUISITION; COLLISION-INDUCED DISSOCIATION; LABEL-FREE QUANTIFICATION; TOP-DOWN PROTEOMICS; HIGH-RESOLUTION; TARGETED ANALYSIS; ACCURATE MASS; LC-MS; QUANTITATIVE PROTEOMICS; PEPTIDE IDENTIFICATION;
D O I
10.1146/annurev-anchem-071015-041535
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Proteins constitute a key class of molecular components that perform essential biochemical reactions in living cells. Whether the aim is to extensively characterize a given protein or to perform high-throughput qualitative and quantitative analysis of the proteome content of a sample, liquid chromatography coupled to tandem mass spectrometry has become the technology of choice. In this review, we summarize the current state of mass spectrometry applied to bottom-up proteomics, the approach that focuses on analyzing peptides obtained from proteolytic digestion of proteins. With the recent advances in instrumentation and methodology, we show that the field is moving away from providing qualitative identification of long lists of proteins to delivering highly consistent and accurate quantification values for large numbers of proteins across large numbers of samples. We believe that this shift will have a profound impact for the field of proteomics and life science research in general.
引用
收藏
页码:449 / 472
页数:24
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