Using Photonic Crystal Enhanced Fluorescence on Quartz Substrates to Improve the Sensitivity of DNA Microarrays

被引：0

作者：

George, Sherine ^{[1
,2
]}

Pokhriyal, Anusha ^{[1
,2
]}

Jones, Sarah I. ^{[3
]}

Lu, Meng

Vodkin, Lila O. ^{[3
]}

Cunningham, Brian T. ^{[1
,4
]}

机构：

[1] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA

来源：

2011 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2011年

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Gene expression analysis of low abundance genes remains difficult when DNA microarrays are performed on standard glass substrates. However, we have shown that by using photonic crystals (PC) made on quartz substrates, the fluorescence intensity of Cyanine-5 (Cy5) labeled microarray spots is greatly enhanced. In a 1-color microarray experiment studying gene expression of soybean cotyledon tissue, an average signal enhancement factor of 17.8x was observed on the PC. Furthermore, twice as many genes were detectable on these PCs as compared to glass. By improving the sensitivity of this fluorescent assay, low expression genes that were undetectable on glass were quantified on the PC.

引用

页码：26 / 29

页数：4

共 7 条

[1] Vapor-Phase Deposition of Monofunctional Alkoxysilanes for Sub-Nanometer-Level Biointerfacing on Silicon Oxide Surfaces [J].

Dorvel, Brian ;

Reddy, Bobby, Jr. ;

Block, Ian ;

Mathias, Patrick ;

Clare, Susan E. ;

Cunningham, Brian ;

Bergstrom, Donald E. ;

Bashir, Rashid .

ADVANCED FUNCTIONAL MATERIALS, 2010, 20 (01) :87-95

[2] Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement biosensors [J].

Ganesh, Nikhil ;

Block, Ian D. ;

Mathias, Patrick C. ;

Zhang, Wei ;

Chow, Edmond ;

Malyarchuk, Viktor ;

Cunningham, Brian T. .

OPTICS EXPRESS, 2008, 16 (26) :21626-21640

[3] Distance dependence of fluorescence enhancement from photonic crystal surfaces [J].

Ganesh, Nikhil ;

Mathias, Patrick C. ;

Zhang, Wei ;

Cunningham, Brian T. .

JOURNAL OF APPLIED PHYSICS, 2008, 103 (08)

[4] Specific elements of the glyoxylate pathway play a significant role in the functional transition of the soybean cotyledon during seedling development [J].

Gonzalez, Delkin O. ;

Vodkin, Lila O. .

BMC GENOMICS, 2007, 8 (1)

[5] Nanoroughened plasmonic films for enhanced biosensing detection [J].

Le Moal, Eric ;

Leveque-Fort, Sandrine ;

Potier, Marie-Claude ;

Fort, Emmanuel .

NANOTECHNOLOGY, 2009, 20 (22)

[6] Employing two distinct photonic crystal resonances to improve fluorescence enhancement [J].

Mathias, Patrick C. ;

Wu, Hsin-Yu ;

Cunningham, Brian T. .

APPLIED PHYSICS LETTERS, 2009, 95 (02)

[7] Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection [J].

Pokhriyal, Anusha ;

Lu, Meng ;

Chaudhery, Vikram ;

Huang, Cheng-Sheng ;

Schulz, Stephen ;

Cunningham, Brian T. .

OPTICS EXPRESS, 2010, 18 (24) :24793-24808

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