Using Photonic Crystal Enhanced Fluorescence on Quartz Substrates to Improve the Sensitivity of DNA Microarrays

被引：0

作者：

George, Sherine ^{[1
,2
]}

Pokhriyal, Anusha ^{[1
,2
]}

Jones, Sarah I. ^{[3
]}

Lu, Meng

Vodkin, Lila O. ^{[3
]}

Cunningham, Brian T. ^{[1
,4
]}

机构：

[1] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA

来源：

2011 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2011年

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Gene expression analysis of low abundance genes remains difficult when DNA microarrays are performed on standard glass substrates. However, we have shown that by using photonic crystals (PC) made on quartz substrates, the fluorescence intensity of Cyanine-5 (Cy5) labeled microarray spots is greatly enhanced. In a 1-color microarray experiment studying gene expression of soybean cotyledon tissue, an average signal enhancement factor of 17.8x was observed on the PC. Furthermore, twice as many genes were detectable on these PCs as compared to glass. By improving the sensitivity of this fluorescent assay, low expression genes that were undetectable on glass were quantified on the PC.

引用

页码：26 / 29

页数：4

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