DNA-PAINT MINFLUX nanoscopy

被引：53

作者：

Ostersehlt, Lynn M. ^{[1
]}

Jans, Daniel C. ^{[1
,2
]}

Wittek, Anna ^{[1
,2
]}

Keller-Findeisen, Jan ^{[1
]}

Inamdar, Kaushik ^{[1
,2
]}

Sahl, Steffen J. ^{[1
]}

Hell, Stefan W. ^{[1
,3
,4
]}

Jakobs, Stefan ^{[1
,2
,4
,5
]}

机构：

[1] Max Planck Inst Multidisciplinary Sci, Dept NanoBiophoton, Gottingen, Germany

[2] Univ Med Ctr Gottingen, Dept Neurol, Gottingen, Germany

[3] Max Planck Inst Med Res, Dept Opt Nanoscopy, Heidelberg, Germany

[4] Univ Gottingen, Cluster Excellence Multiscale Bioimaging Mol Mach, Gottingen, Germany

[5] Fraunhofer Inst Translat Med & Pharmacol ITMP, Translat Neuroinflammat & Automated Microscopy, Gottingen, Germany

来源：

NATURE METHODS | 2022年 / 19卷 / 09期

关键词：

MICROSCOPY; RESOLUTION; BINDING;

D O I：

10.1038/s41592-022-01577-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells. A systematic exploration of MINFLUX nanoscopy with DNA-PAINT labeling leads to improved nanoscopy in fixed cells and MINFLUX imaging with increased multiplexing, as exemplified by three-color imaging of mitochondria in mammalian cells.

引用

页码：1072 / +

页数：13

共 25 条

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