Profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography

被引:29
作者
Mendiola, Jose A. [1 ]
Marin, Francisco R. [2 ]
Senorans, F. Javier [2 ]
Reglero, Guillermo [2 ]
Martin, Pedro J. [1 ]
Cifuentes, Alejandro [1 ]
Ibanez, Elena [1 ]
机构
[1] CSIC, Inst Fermentac Ind, E-28006 Madrid, Spain
[2] Univ Autonoma Madrid, Secc Dept Ciencias Alimentac, Unidad Asociada, CSIC, E-28049 Madrid, Spain
关键词
functional foods; water-soluble vitamins; fat-soluble vitamins; phenolic compounds; HPLC-DAD; Folin-Ciocalteu; antioxidant activity; PLS;
D O I
10.1016/j.chroma.2008.02.054
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the present work, an HPLC method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated C18 column and gradient separation using trifluoroacetic acid, water and methanol. It is shown that the HPLC method provides baseline separation of all these compounds with good resolution values in 40 min. Moreover, other figures of merit of the method show a good linear response and low detection limits for all the compounds considered in the present study. Furthermore, the usefulness of this method is demonstrated via its successful application to the analysis of different beverages from different natural origin (orange, strawberry, apple, peach pineapple, plum and blackcurrant juices, soybean milk, beers) without the need of any previous sample preparation. A good correlation is also found by comparing the total phenol content (measured by Folin-Ciocalteu method) with the sum of total phenolic compounds obtained using the proposed HPLC method. By using statistical tools, the main compounds associated with antioxidant activity of the extracts (measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging) were assessed. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:234 / 241
页数:8
相关论文
共 51 条
[1]   Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material [J].
Almela, Luis ;
Sanchez-Munoz, Blas ;
Fernandez-Lopez, Jose A. ;
Roca, Maria J. ;
Rabe, Virginia .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1120 (1-2) :221-229
[2]  
Beecher G, 1997, FASEB J, V11, P2589
[3]   Antioxidant activity of phenolics extracted from Olea europaea L-leaves [J].
Benavente-García, O ;
Castillo, J ;
Lorente, J ;
Ortuño, A ;
Del Rio, JA .
FOOD CHEMISTRY, 2000, 68 (04) :457-462
[4]   Fast and simultaneous detection of prominent natural antioxidants using analytical microsystems for capillary electrophoresis with a glassy carbon electrode:: A new gateway to food environments [J].
Blasco, AJ ;
Barrigas, I ;
González, MC ;
Escarpa, A .
ELECTROPHORESIS, 2005, 26 (24) :4664-4673
[5]   Antioxidant activity and phenolic composition of citrus peel and seed extracts [J].
Bocco, A ;
Cuvelier, ME ;
Richard, H ;
Berset, C .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (06) :2123-2129
[7]   Simultaneous determination of the vitamins A, E, their esters and coenzyme Q10 in multivitamin dietary supplements using an RP-C30 phase [J].
Breithaupt, DE ;
Kraut, S .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2006, 222 (5-6) :643-649
[8]   LC/UV/MS-MRM for the simultaneous determination of water-soluble vitamins in multi-vitamin dietary supplements [J].
Chen, Pei ;
Wolf, Wayne R. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (07) :2441-2448
[9]   Influence of heat treatment and grain germination on the isoflavone profile of soy milk [J].
Chiarello, MD ;
Le Guerroué, JL ;
Chagas, CMS ;
Franco, OL ;
Bianchini, E ;
Joao, MJ .
JOURNAL OF FOOD BIOCHEMISTRY, 2006, 30 (02) :234-247
[10]   Simultaneous determination of water-soluble vitamins excreted in human urine after eating an overdose of vitamin pills by a HPLC method coupled with a solid phase extraction [J].
Cho, CM ;
Ko, JH ;
Cheong, WJ .
TALANTA, 2000, 51 (04) :799-806