SERS labels for quantitative assays: application to the quantification of gold nanoparticles uptaken by macrophage cells

被引：24

作者：

Amendola, Vincenzo ^{[1
]}

Meneghetti, Moreno ^{[1
]}

Fiameni, Stefania ^{[2
]}

Polizzi, Stefano ^{[3
,4
]}

Fracasso, Giulio ^{[5
]}

Boscaini, Anita ^{[5
]}

Colombatti, Marco ^{[5
]}

机构：

[1] Univ Padua, Dept Chem Sci, I-35131 Padua, Italy

[2] CNR IENI, I-35127 Padua, Italy

[3] Univ Ca Foscari Venezia, Dept Mol Sci & Nanosyst, I-30172 Venice, Italy

[4] INSTM UdR Venezia, I-30172 Venice, Italy

[5] Univ Verona, Immunol Sect, Dept Pathol & Diagnost, I-37134 Verona, Italy

来源：

ANALYTICAL METHODS | 2011年 / 3卷 / 04期

关键词：

ENHANCED RAMAN-SCATTERING; ORGANIC-INORGANIC NANOPARTICLES; IN-VIVO; SPECTRAL-ANALYSIS; SINGLE-MOLECULE; LASER-ABLATION; SILVER; SPECTROSCOPY; TAGS; DNA;

D O I：

10.1039/c0ay00660b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Labels based on noble metal nanoparticles and surface enhanced Raman scattering (SERS) opened new opportunities for the ultrasensitive detection of analytes. To date, however, SERS labels were mostly used for qualitative analysis, while leaving largely unexploited their potential for ultrasensitive quantitative assays. Here we synthesized SERS labels based on gold nanoparticles (AuNPs) obtained by laser ablation synthesis in solution and we developed a general method for the correlation of the SERS label concentration with the intensity of the Raman signal. We successfully used this method for the quantification of the number of AuNPs uptaken by PMA differentiated U937 macrophage cells. Our work shows that quantitative ultrasensitive assays by SERS labels are possible and points out some issues that must be considered when performing this type of analysis.

引用

页码：849 / 856

页数：8

共 57 条

[41] In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags [J].