Molecular characterisation and genetic analysis of aerolysin and haemolysin in Aeromonas hydrophila isolated from diseased Labeo rohita by polymerase chain reaction

被引：1

作者：

Sughra, Fatima ^{[1
]}

Hafeez-ur-Rehman, Muhammad ^{[2
]}

Abbas, Farzana ^{[2
]}

Altaf, Imran ^{[3
]}

Hassan, Zohal ^{[3
]}

Bhatti, Ayesha ^{[2
]}

Ali, Kashif ^{[4
]}

机构：

[1] Univ Educ, Div Sci & Technol, Dept Zool, Lahore, Pakistan

[2] Univ Vet & Anim Sci, Dept Fisheries & Aquaculture, Lahore, Pakistan

[3] Univ Vet & Anim Sci, Qual Operat & Res Lab, Lahore, Pakistan

[4] Univ Vet & Anim Sci, Dept Wildlife Ecol, Lahore, Pakistan

来源：

JOURNAL OF FISHERIES | 2022年 / 10卷 / 03期

关键词：

aerolysin; Aeromonas hydrophila; DNA; haemolysin; polymerase chain reaction; sequencing; EPIZOOTIC ULCERATIVE SYNDROME; FISH; IDENTIFICATION; SOBRIA;

D O I：

10.17017/j.fish.466

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The present study investigates pathogenicity of local Aeromonas hydrophila strains by molecular characterisation of two virulence factor genes: aerolysin (aerA) and haemolysin (Ahh1) using polymerase chain reaction (PCR) technique. Phenotypically identified presumptive Aeromonas isolates recovered from diseased Labeo rohita were genetically analysed using type-specific primers by amplifying 309 bp and 130 bp conserved regions of aerolysin and haemolysin genes respectively. The partial nucleotide sequences of aerA and Ahh1 were determined from representative strains in which aerA was confirmed in 75% isolates, whereas Ahh1 was confirmed in 50% isolates. The nucleotide blast results of the representative strains revealed close homology of 95% (aerolysin) and 97% (haemolysin) with published sequences.

引用

页数：5

共 22 条

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