Oxidation of carbonyl compounds by whole-cell biocatalyst

被引：2

作者：

Gawai, KR ^{[1
]}

Lokhande, PD ^{[1
]}

Kodam, KM ^{[1
]}

Soojhawon, I ^{[1
]}

机构：

[1] Univ Pune, Dept Chem, Biochem Sect, Pune 411007, Maharashtra, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2005年 / 21卷 / 04期

关键词：

Acinetobacter junii; biocatalyst; biotransformation; cytochrome P-450;

D O I：

10.1007/s11274-004-2467-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Acinetobacter junii was found to catalyse the oxidative biotransformation of benzaldehyde, 4-methoxybenzaldehyde, vanillin, 3,4-dimethoxybenzaldehyde, 3-methoxybenzaldehyde and phthalaldehyde within 48 h of incubation. During this process, the activities of drug-metabolizing enzymes, such as cytochrome P-450 and acetanilide hydroxylase were found to be increased significantly. Such an increase in activity indicates their involvement in the biotransformation processes. The purified biotransformed products of each carbonyl compound were characterized by H-1 NMR and IR spectroscopy, confirming that oxidation to the corresponding carboxylic acid had occurred.

引用

页码：457 / 461

页数：5

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