Optimizing the optical trapping stiffness of holographically trapped microrods using high-speed video tracking

被引：44

作者：

Phillips, D. B. ^{[1
]}

Carberry, D. M. ^{[1
]}

Simpson, S. H. ^{[1
]}

Schaefer, H. ^{[2
]}

Steinhart, M. ^{[2
]}

Bowman, R. ^{[3
]}

Gibson, G. M. ^{[3
]}

Padgett, M. J. ^{[3
]}

Hanna, S. ^{[1
]}

Miles, M. J. ^{[1
]}

机构：

[1] Univ Bristol, HH Wills Phys Lab, Bristol BS8 1TL, Avon, England

[2] Univ Osnabrueck, Inst Chem, D-46069 Osnabruck, Germany

[3] Univ Glasgow, Dept Phys & Astron, SUPA, Glasgow G12 8QQ, Lanark, Scotland

来源：

JOURNAL OF OPTICS | 2011年 / 13卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

optical tweezers; particle tracking; optical trap calibration; TWEEZERS; FORCE; NANOWIRES; MICROMANIPULATION; MANIPULATION; MICROSCOPY; PARTICLES; CELLS;

D O I：

10.1088/2040-8978/13/4/044023

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Dielectric microrods can be trapped horizontally in pairs of holographically controlled optical traps. External forces acting on these microrods are registered via the rotational and translational displacement of the microrod relative to the traps. In the following paper we demonstrate accurate, real-time tracking of this displacement in two dimensions. The precision of the method is estimated and the translational and rotational stiffness coefficients of the trapped microrod are evaluated by analysing the thermal motion and the Stokes drag. The variation of these stiffness coefficients relative to the displacement of the traps from the ends of the microrods is measured, and optimal trapping conditions are located.

引用

页数：8

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