nMAT1, a nuclear-encoded maturase involved in the trans-splicing of nad1 intron 1, is essential for mitochondrial complex I assembly and function

被引：108

作者：

Keren, Ido ^{[1
,2
]}

Tal, Liat ^{[1
,2
]}

des Francs-Small, Catherine C. ^{[3
]}

Araujo, Wagner L. ^{[4
]}

Shevtsov, Sofia ^{[1
]}

Shaya, Felix ^{[1
]}

Fernie, Alisdair R. ^{[4
]}

Small, Ian ^{[3
]}

Ostersetzer-Biran, Oren ^{[1
]}

机构：

[1] Agr Res Org ARO, Volcani Ctr, Inst Plant Sci, IL-50250 Bet Dagan, Israel

[2] Hebrew Univ Jerusalem, Dept Plant Sci, IL-76100 Rehovot, Israel

[3] Univ Western Australia, Australian Res Council ARC, Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia

[4] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

来源：

PLANT JOURNAL | 2012年 / 71卷 / 03期

基金：

美国国家科学基金会; 澳大利亚研究理事会;

关键词：

maturase; group II; intron; splicing; mitochondria; Arabidopsis; COMPLETE NUCLEOTIDE-SEQUENCE; HIGHER-PLANT MITOCHONDRIA; ELECTRON-TRANSPORT CHAIN; ARABIDOPSIS-THALIANA; REVERSE TRANSCRIPTASE/MATURASE; NICOTIANA-SYLVESTRIS; METABOLISM; PROTEIN; GENE; GENOME;

D O I：

10.1111/j.1365-313X.2012.04998.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Mitochondrial genomes (mtDNAs) in angiosperms contain numerous group II-type introns that reside mainly within protein-coding genes that are required for organellar genome expression and respiration. While splicing of group II introns in non-plant systems is facilitated by proteins encoded within the introns themselves (maturases), the mitochondrial introns in plants have diverged and have lost the vast majority of their intron-encoded ORFs. Only a single maturase gene (matR) is retained in plant mtDNAs, but its role(s) in the splicing of mitochondrial introns is currently unknown. In addition to matR, plants also harbor four nuclear maturase genes (nMat 1 to 4) encoding mitochondrial proteins that are expected to act in the splicing of group II introns. Recently, we established the role of one of these proteins, nMAT2, in the splicing of several mitochondrial introns in Arabidopsis. Here, we show that nMAT1 is required for trans-splicing of nad1 intron 1 and also functions in cis-splicing of nad2 intron 1 and nad4 intron 2. Homozygous nMat1 plants show retarded growth and developmental phenotypes, modified respiration activities and altered stress responses that are tightly correlated with mitochondrial complex I defects.

引用

页码：413 / 426

页数：14

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