Profiling of Antibody Production against Xenograft-released Proteins by Protein Microarrays Discovers Prostate Cancer Markers

被引：12

作者：

Jansen, Flip H.

van Rijswijk, Angelique

Teubel, Wilma

van Weerden, Wytske M.

Reneman, Suzanne

van den Bemd, Gert-Jan

Roobol, Monique J.

Bangma, Chris H.

Staal, Frank J. T. ^{[2
]}

Jenster, Guido ^{[1
]}

机构：

[1] Erasmus MC, Josephine Nefkens Inst, Dept Urol, NL-3000 CA Rotterdam, Netherlands

[2] Erasmus MC, Dept Immunol, NL-3000 CA Rotterdam, Netherlands

来源：

JOURNAL OF PROTEOME RESEARCH | 2012年 / 11卷 / 02期

关键词：

prostate cancer; biomarkers; xenograft; protoarray; TYROSINE KINASE; MODEL SYSTEM; SERUM; IDENTIFICATION; PROTEASOME; BIOMARKER; ANTIGEN;

D O I：

10.1021/pr2006473

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study describes a novel xenograft-based biomarker discovery platform and proves its usefulness in the discovery of serum markers for prostate cancer. By immunizing immuno-competent mice with serum from nude mice bearing prostate cancer xenografts, an antibody response against xenograft-derived antigens was elicited. By probing protein microarrays with serum from immunized mice, several prostate cancer-derived antigens were identified, of which a subset was successfully retrieved in serum from mice bearing prostate cancer xenografts and prevalidated in human serum samples of prostate cancer patients. Among the discovered and validated proteins were the members of the TAM receptor family (TYRO3, AXL, and MERTK), ACY1, and PSMA1. In conclusion, this novel method allows for the identification of low abundant cancer-derived serum proteins, circumventing dynamic range and host-response issues in standard patient cohort proteomics comparisons.

引用

页码：728 / 735

页数：8

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