Sequence Analysis and Heterologous Expression of Polygalacturonase Gene (AspecA) from a Shoyu Koji Mold, Aspergillus sojae KBN1340

被引：8

作者：

Yoshino-Yasuda, Shoko ^{[1
]}

Kato, Masashi ^{[2
]}

Kitamoto, Noriyuki ^{[1
]}

机构：

[1] Aichi Ind Technol Inst, Food Res Ctr, Nishi Ku, Nagoya, Aichi 4510083, Japan

[2] Meijo Univ, Fac Agr, Dept Appl Biol Chem, Tempaku Ku, Nagoya, Aichi 4688502, Japan

来源：

FOOD SCIENCE AND TECHNOLOGY RESEARCH | 2011年 / 17卷 / 06期

关键词：

shoyu koji mold; Aspergillus sojae; polygalacturonase gene; overexpression; SITE-DIRECTED MUTAGENESIS; ENDOPOLYGALACTURONASE II; ORYZAE KBN616; NIGER; FUMIGATUS; FLAVUS;

D O I：

10.3136/fstr.17.579

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A polygalacturonase gene designated as AspecA was isolated from a shoyu koji mold, Aspergillus sojae KBN1340, and characterized. The structural gene comprised 1,226 bp with two introns. The open reading frame encoded a 363-residue protein with a signal peptide of 28 residues. AspecA shared a high degree of sequence identity with Aspergillus parasiticus pecA, Aspergillus oryzae pgaA and Aspergillus flavus pecA. Utilizing the promoter of the A. oryzae taaG2 gene and its signal peptide, AsPecA was successfully expressed in A. oryzae and secreted into the culture medium. AsPecA had a molecular mass of 36.0 kDa, a pH optimum of 5.0, and a temperature optimum of 50 degrees C.

引用

页码：579 / 584

页数：6

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