17β-Estradiol attenuates saturated fatty acid diet-induced liver injury in ovariectomized mice by up-regulating hepatic senescence marker protein-30

被引：22

作者：

Fukui, Michiaki ^{[1
]}

Senmaru, Takafumi

Hasegawa, Goji

Yamazaki, Masahiro

Asano, Mai

Kagami, Yayoi ^{[4
]}

Ishigami, Akihito ^{[4
]}

Maruyama, Naoki ^{[4
]}

Iwasa, Koichi ^{[2
]}

Kitawaki, Jo ^{[2
]}

Itoh, Yoshito ^{[3
]}

Okanoue, Takeshi ^{[5
]}

Ohta, Mitsuhiro ^{[6
]}

Obayashi, Hiroshi ^{[7
]}

Nakamura, Naoto

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Endocrinol & Metab, Kamikyo Ku, Kyoto 6028566, Japan

[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Obstet & Gynecol, Kyoto 6028566, Japan

[3] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Mol Gastroenterol & Hepatol, Kyoto 6028566, Japan

[4] Tokyo Metropolitan Inst Gerontol, Tokyo, Japan

[5] Saiseikai Salta Hosp, Dept Gastroenterol & Hepatol, Osaka, Japan

[6] Kobe Pharmaceut Univ, Dept Med Biochem, Kobe, Hyogo 658, Japan

[7] Inst Bioresponse Informat, Kyoto, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 415卷 / 02期

基金：

日本学术振兴会;

关键词：

Senescence marker protein-30; 17; beta-Estradiol; Endoplasmic reticulum stress; Apoptosis; Saturated fatty acids; Nonalcoholic steatohepatitis; ENDOPLASMIC-RETICULUM STRESS; TERM EZETIMIBE THERAPY; INDUCED APOPTOSIS; GENE-EXPRESSION; DISEASE; SMP30; STEATOSIS; CALCIUM; ALPHA; CELLS;

D O I：

10.1016/j.bbrc.2011.10.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Senescence marker protein-30 (SMP30) plays an important role in intracellular Ca2+ homeostasis. The aim of the present study was to investigate the effects of estrogens on liver apoptotic damage and changes in SMP30 expression induced by a high saturated fatty acid diet (HSFD). Ovariectomized mice (OVX) and sham-operated mice (SHAM) were randomly divided into five groups: SHAM fed a normal diet (SHAM/ND). SHAM fed HSFD (SHAM/HSFD), OVX fed ND (OVX/ND), OVX fed HSFD (OVX/HSFD) and OVX fed HSFD with 17 beta-estradiol (E2) supplementation using an implanted slow-release pellet (OVX/ HSFD + E2). After 8 weeks, markers of endoplasmic reticulum (ER) stress and apoptosis, and levels of tumor necrosis factor-alpha (TNF alpha and SMP30 expression were investigated. Compared with SHAM/ND, OVX/HSFD mice showed significantly increased spliced X-box protein-1 (s-XBP1), phosphorylated eukaryotic initiation factor-2 alpha (p-eIF2 alpha), glucose-regulated protein 78 (GPR78), C/EBP homologous protein (CHOP), cytosolic cytochrome c, caspase-3 activity, and TNF alpha, and significantly decreased SMP30. These differences in OVX/HSFD mice were restored to the levels of SHAM/ND mice by E2 supplementation. These results suggest that E2 supplementation attenuates HSFD-induced liver apoptotic death in ovariectomized mice by up-regulating SMP30. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：252 / 257

页数：6

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