Identification of an alkaline lipase capable of better enrichment of EPA than DHA due to fatty acids selectivity and regioselectivity

被引：33

作者：

Gao, Kunpeng ^{[1
]}

Chu, Wenqin ^{[1
]}

Sun, Jianan ^{[1
]}

Mao, Xiangzhao ^{[1
,2
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China

来源：

FOOD CHEMISTRY | 2020年 / 330卷

基金：

中国国家自然科学基金;

关键词：

Streptomyces violascens; Genome sequence; Lipase; EPA; Fish oil; Fatty acids selectivity; Regioselectivity; FISH-OIL; EICOSAPENTAENOIC ACID; STREPTOMYCES-VIOLASCENS; TRIGLYCERIDES; MODEL; PUFA;

D O I：

10.1016/j.foodchem.2020.127225

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The whole genome of Streptomyces violascens (=ATCC 27968) was sequenced and the cloning and expression of OUC-Lipase 6 were conducted in Bacillus subtilis WB800. The recombinant enzyme belongs to the lipolytic enzymes family V. OUC-Lipase 6 showed optimal activity at 30 degrees C and pH 9.0, and retained 90.2% of its activity in an alkaline buffer (pH 8.0, 30 degrees C and 96 h). OUC-Lipase 6 showed good stability under medium temperature conditions (residual activity of 68.8%, pH 8.0, 45 degrees C and 96 h). OUC-Lipase 6 could selectively hydrolyze fatty acids on the glyceride backbone, thus improving the contents of DHA and EPA in codfish oil. OUC-Lipase 6 also showed regioselectivity, resulting in a better enrichment efficiency for EPA than DHA. After hydrolyzing for 36 h via OUC-Lipase 6, the contents of EPA and DHA were improved to 3.24-fold and 1.98-fold, respectively.

引用

页数：9

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