In situ localization of avenanthramide A and its biosynthetic enzyme in oat leaves infected with the crown rust fungus, Puccinia coronata f. sp avenae

被引：6

作者：

Uchihashi, Kohhei ^{[1
]}

Nakayashiki, Hitoshi ^{[1
]}

Okamura, Kaoru ^{[1
]}

Ishihara, Atsushi ^{[2
]}

Tosa, Yukio ^{[1
]}

Park, Pyoyun ^{[1
]}

Mayama, Shigeyuki ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Agr Sci, Plant Pathol Lab, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Tottori Univ, Fac Agr, Tottori 6808550, Japan

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2011年 / 76卷 / 3-4期

关键词：

Avenanthramide A; Phytoalexin biosynthetic enzyme; PR-10; Oat; Puccinia coronata f. sp avenae; PHYTOALEXINS; PROTEINS; AVENALUMIN; RESISTANCE; INDUCTION; ELICITOR;

D O I：

10.1016/j.pmpp.2011.09.004

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We determined the temporal and spatial localization of the phytoalexin avenanthramide A, and its biosynthetic enzyme, hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroxy-cinnamoyltransferase (AsHHT) in oat leaves infected with the crown rust fungus. Accumulation of avenanthramide A and AsHHT was first observed predominantly in hypersensitive response (HR) cells at 36 h post inoculation (hpi), and later in adjacent cells at 48 hpi. At 120 hpi, avenanthramide A was detected in a wider area of infected tissues, but AsHHT-positive signals were only observed in the HR and adjacent cells, suggesting that avenanthramide A was synthesized around the HR cells, and then transported to other parts of the infected tissues in a center-to-periphery manner. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：173 / 181

页数：9

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