New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks

被引：20

作者：

Li, Cai-xia ^{[1
,2
]}

Wang, Gui-qiang ^{[1
,2
]}

Li, Wan-shui ^{[1
,2
]}

Huang, Jiang-ping ^{[3
]}

Ji, An-quan ^{[1
,2
]}

Hu, Lan ^{[1
,2
]}

机构：

[1] Minist Publ Secur, Inst Forens Sci, Beijing 100038, Peoples R China

[2] Minist Publ Secur, Key Lab Forens Genet, Beijing 100038, Peoples R China

[3] Chinese Peoples Publ Secur Univ, Beijing, Peoples R China

来源：

CROATIAN MEDICAL JOURNAL | 2011年 / 52卷 / 03期

关键词：

IN-SITU HYBRIDIZATION; LASER MICRODISSECTION; EPITHELIAL-CELLS; SEXUAL ASSAULT; DNA; SPERMATOZOA; RECOVERY;

D O I：

10.3325/cmj.2011.52.293

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Aim To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis. Methods To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments. Results and conclusions We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective forensic tool for the analysis of specific cells from complex samples.

引用

页码：293 / 298

页数：6

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