A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells

被引：39
|
作者：
Jiang, Yin [1 ]
Zheng, Guansheng [1 ]
Cai, Ning [1 ]
Zhang, Huatang [1 ]
Tan, Yi [2 ]
Huang, Mengjiao [1 ]
He, Yonghe [1 ]
He, Jun [1 ]
Sun, Hongyan [2 ,3 ]
机构：
[1] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China
[2] City Univ Hong Kong, Dept Chem, 83 Tat Chee Ave, Kowloon, Hong Kong, Peoples R China
[3] City Univ Hong Kong, Shenzhen Res Inst, Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen 518057, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 91期
基金：
中国国家自然科学基金;
关键词：
OXIDATIVE STRESS; PARKINSONS-DISEASE; IN-VIVO; FREE-RADICALS; CANCER-CELLS; LIVE CELLS; MYELOPEROXIDASE; MECHANISMS; ROS; MITOCHONDRIA;
D O I：
10.1039/c7cc07373a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A facile fluorescent probe (NBD-DOP) has been developed to detect hypochlorous acid (HOCl) in this study. The probe consists of a NBD fluorophore and a dopamine moiety that reacts with HOCl specifically. The dopamine group quenches the fluorescence of NBD efficiently through a photoinduced electron transfer (PET) effect. Experimental data showed that NBD-DOP could detect HOCl with ultrafast response, high sensitivity and high selectivity in a wide pH range. The probe could also be used to detect the myeloperoxidase enzyme that produces HOCl. Moreover, NBD-DOP has been applied in the imaging of exogenous and endogenous HOCl in living cells by confocal fluorescence microscopy.
引用
收藏
页码：12349 / 12352
页数：4
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