A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells

被引：40

作者：

Jiang, Yin ^{[1
]}

Zheng, Guansheng ^{[1
]}

Cai, Ning ^{[1
]}

Zhang, Huatang ^{[1
]}

Tan, Yi ^{[2
]}

Huang, Mengjiao ^{[1
]}

He, Yonghe ^{[1
]}

He, Jun ^{[1
]}

Sun, Hongyan ^{[2
,3
]}

机构：

[1] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China

[2] City Univ Hong Kong, Dept Chem, 83 Tat Chee Ave, Kowloon, Hong Kong, Peoples R China

[3] City Univ Hong Kong, Shenzhen Res Inst, Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen 518057, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 91期

基金：

中国国家自然科学基金;

关键词：

OXIDATIVE STRESS; PARKINSONS-DISEASE; IN-VIVO; FREE-RADICALS; CANCER-CELLS; LIVE CELLS; MYELOPEROXIDASE; MECHANISMS; ROS; MITOCHONDRIA;

D O I：

10.1039/c7cc07373a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A facile fluorescent probe (NBD-DOP) has been developed to detect hypochlorous acid (HOCl) in this study. The probe consists of a NBD fluorophore and a dopamine moiety that reacts with HOCl specifically. The dopamine group quenches the fluorescence of NBD efficiently through a photoinduced electron transfer (PET) effect. Experimental data showed that NBD-DOP could detect HOCl with ultrafast response, high sensitivity and high selectivity in a wide pH range. The probe could also be used to detect the myeloperoxidase enzyme that produces HOCl. Moreover, NBD-DOP has been applied in the imaging of exogenous and endogenous HOCl in living cells by confocal fluorescence microscopy.

引用

页码：12349 / 12352

页数：4

共 46 条

[1] A highly specific ferrocene-based fluorescent probe for hypochlorous acid and its application to cell imaging [J].

Chen, Suming ;

Lu, Jinxin ;

Sun, Chengdong ;

Ma, Huimin .

ANALYST, 2010, 135 (03) :577-582

[2] Oxidative stress in neurodegenerative diseases [J].

Chen, Xueping ;

Guo, Chunyan ;

Kong, Jiming .

NEURAL REGENERATION RESEARCH, 2012, 7 (05) :376-385

[3] ROS as signalling molecules:: mechanisms that generate specificity in ROS homeostasis [J].

D'Autreaux, Benoit ;

Toledano, Michel B. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (10) :813-824

[4] Myeloperoxidase-derived oxidation: mechanisms of biological damage and its prevention [J].

Davies, Michael J. .

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 2011, 48 (01) :8-19

[5] Molecular pathways of neurodegeneration in Parkinson's disease [J].

Dawson, TM ;

Dawson, VL .

SCIENCE, 2003, 302 (5646) :819-822

[6] Free radicals in the physiological control of cell function [J].

Dröge, W .

PHYSIOLOGICAL REVIEWS, 2002, 82 (01) :47-95

[7] Targeting reactive oxygen species in development and progression of pancreatic cancer [J].

Durand, Nisha ;

Storz, Peter .

EXPERT REVIEW OF ANTICANCER THERAPY, 2017, 17 (01) :19-31

[8] Signal transduction by reactive oxygen species [J].

Finkel, Toren .

JOURNAL OF CELL BIOLOGY, 2011, 194 (01) :7-15

[9] Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque [J].

Fu, S ;

Davies, MJ ;

Stocker, R ;

Dean, RT .

BIOCHEMICAL JOURNAL, 1998, 333 :519-525

[10] HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay [J].

Hu, Jun Jacob ;

Wong, Nai-Kei ;

Lu, Ming-Yang ;

Chen, Xingmiao ;

Ye, Sen ;

Zhao, Angela Qian ;

Gao, Peng ;

Kao, Richard Yi-Tsun ;

Shen, Jiangang ;

Yang, Dan .

CHEMICAL SCIENCE, 2016, 7 (03) :2094-2099

← 1 2 3 4 5 →