Milk Allergen Detection: Sensitive Label-Free Voltammetric Immunosensor Based on Electropolymerization

被引：9

作者：

Kilic, Tugba ^{[1
]}

Philipp, Paul Jean ^{[1
]}

Giavedoni, Paola ^{[2
]}

Carrara, Sandro ^{[1
]}

机构：

[1] Swiss Fed Inst Technol Lausanne, Lausanne, Switzerland

[2] Grp Elsa Mifroma MIGROS, Estavayer Le Lac, Estavayer, Switzerland

来源：

BIONANOSCIENCE | 2020年 / 10卷 / 02期

关键词：

Alpha lactalbumin; Milk allergens; Food allergy; Pyrrole; Electropolymerization; Immunosensor; ALPHA-LACTALBUMIN; BIOSENSOR; ANTIBODY; PLATFORM;

D O I：

10.1007/s12668-020-00730-4

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

We present in this work, first time in literature, a voltammetric label-free biosensor for detection of alpha lactalbumin (alpha-LB) in meal samples. Food allergy concern necessitates strict regulations in food labelling and innovative monitoring strategies for prevention of sepsis-related death. As major food allergens, dietary samples often contain whey proteins, including alpha-LB as one of the key allergenic. Available detection methods for alpha-LB are based on successive labelling steps and hence have long assay times with limited sensitivity. The new detection method presented here is based on capturing of alpha-LB via entrapped alpha-LB antibody (alpha-LB-Ab) through electropolymerization of polypyrrole (PPy) and then measuring the conductivity decrease by differential pulse voltammetry (DPV). This new label-free voltammetric biosensor for alpha-LB exhibits the best limit of detection (0.19 fg/mL) published so far in literature, with high selectivity and capability to quantify alpha-LB in real milk samples too.

引用

页码：512 / 522

页数：11

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