A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity

被引:39
作者
Juanes-Garcia, Alba [1 ]
Chapman, Jessica R. [2 ]
Aguilar-Cuenca, Rocio [1 ]
Delgado-Arevalo, Cristina [1 ]
Hodges, Jennifer [4 ]
Whitmore, Leanna A. [4 ]
Shabanowitz, Jeffrey [2 ]
Hunt, Donald F. [2 ,3 ]
Horwitz, Alan Rick [4 ]
Vicente-Manzanares, Miguel [1 ]
机构
[1] Univ Autonoma Madrid, Sch Med, Madrid 28006, Spain
[2] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA
[3] Univ Virginia, Dept Pathol, Charlottesville, VA 22901 USA
[4] Univ Virginia, Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA
关键词
PHOSPHORYLATION; KINASE; LOCALIZATION; ACTOMYOSIN; ACTIN; ACTIVATION; CELLS; CDC42; PAK1; MRCK;
D O I
10.1083/jcb.201407059
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this study, we show that the role of nonmuscle myosin II (NMII)-B in front-back migratory cell polarity is controlled by a short stretch of amino acids containing five serines (1935-1941). This motif resides near the junction between the C terminus helical and nonhelical tail domains. Removal of this motif inhibited NMII-B assembly, whereas its insertion into NMII-A endowed an NMII-B-like ability to generate large actomyosin bundles that determine the rear of the cell. Phosphomimetic mutation of the five serines also inhibited NMII-B assembly, rendering it unable to support front-back polarization. Mass spectrometric analysis showed that several of these serines are phosphorylated in live cells. Single-site mutagenesis showed that serine 1935 is a major regulatory site of NMII-B function. These data reveal a novel regulatory mechanism of NMII in polarized migrating cells by identifying a key molecular determinant that confers NMII isoform functional specificity.
引用
收藏
页码:23 / 32
页数:10
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