Effect of active immunization against a recombinant mouse granulocyte-macrophage colony-stimulating factor/somatostatin fusion protein on the growth of mice

被引：6

作者：

Wu, Bingbing ^{[1
]}

Qi, Ruili ^{[1
]}

Li, Bin ^{[1
]}

Yuan, Taoyan ^{[1
]}

Liu, Heshan ^{[1
]}

He, Jun ^{[1
]}

Lin, Zhiwei ^{[1
]}

Li, Weifen ^{[1
]}

Fu, Yan ^{[1
]}

Niu, Dong ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 06期

关键词：

Somatostatin; Granulocyte-macrophage colony-stimulating factor; Active immunization; Growth hormone; IGF-1; BODY-COMPOSITION; GROWING-PIGS; HORMONE; SOMATOSTATIN; MYOSTATIN; VACCINATION; METABOLISM; EXPRESSION; SECRETION;

D O I：

10.1007/s11033-012-1502-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Female BALB/c mice were actively immunized subcutaneously with a recombinant protein of granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with somatostatin (SS) (GM-CSF/SS). Fifty-four days after the primary immunization, the body weight of the immunized mice increased by 4.62% compared with the control (P < 0.05), together with the induction of detectable serum antibodies against SS. The level of serum growth hormone (GH) elevated by 44.54% (P < 0.05) and the mRNA expression of muscular IGF-1 increased by 94% for the GM-CSF/SS-treated mice. The results indicated that the recombinant protein GM-CSF/SS was efficient in inducing specific immunity against SS, subsequently leading to the increase of the GH level by SS neutralization, and ultimately improving the growth of mice.

引用

页码：6773 / 6779

页数：7

共 30 条

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