Isolation and characterization of Vibrio parahaemolyticus causing infection in Iberian toothcarp Aphanius iberus

被引:46
作者
Alcaide, E [1 ]
Amaro, C [1 ]
Todolí, R [1 ]
Oltra, R [1 ]
机构
[1] Univ Valencia, Dept Microbiol & Ecol, Fac Ciencias Biol, E-46100 Valencia, Spain
关键词
Vibrio parahaemolyticus; vibriosis; fish disease; Aphanius iberus;
D O I
10.3354/dao035077
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
High mortality among laboratory cultured Iberlan toothcarp Aphanius iberus occurred in February 1997 in Valencia (Spain). The main signs of the disease were external haemorrhage and tail rot. Bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as Vibrio parahaemolyticus. The bacteria were haemolytic against erythrocytes from eel Anguilla anguilla, amberjack Seriola dumerili, toothcarp A. iberus and humans, and were Kanagawa-phenomenon-negative. Infectivity tests showed that the virulence for A. iberus was dependent on salinity. Finally, all strains were virulent for amberjack and eel.
引用
收藏
页码:77 / 80
页数:4
相关论文
共 14 条
[1]   COMPARATIVE-STUDY OF PHENOTYPIC AND VIRULENCE PROPERTIES IN VIBRIO-VULNIFICUS BIOTYPE-1 AND BIOTYPE-2 OBTAINED FROM A EUROPEAN EEL FARM EXPERIENCING MORTALITIES [J].
AMARO, C ;
BIOSCA, EG ;
ESTEVE, C ;
FOUZ, B ;
TORANZO, AE .
DISEASES OF AQUATIC ORGANISMS, 1992, 13 (01) :29-35
[2]  
BLANCO JC, 1992, LIBRO ROJO VERTEBRAD
[3]  
Hjeltnes B., 1993, P109
[4]   The epidemiology of Vibrio infections in Florida, 1981-1993 [J].
Hlady, WG ;
Klontz, KC .
JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (05) :1176-1183
[5]   PURIFICATION AND CHARACTERIZATION OF A HEMOLYSIN PRODUCED BY A CLINICAL ISOLATE OF KANAGAWA PHENOMENON-NEGATIVE VIBRIO-PARAHAEMOLYTICUS AND RELATED TO THE THERMOSTABLE DIRECT HEMOLYSIN [J].
HONDA, T ;
NI, YX ;
MIWATANI, T .
INFECTION AND IMMUNITY, 1988, 56 (04) :961-965
[6]   CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE (TRH) ENCODING THE HEMOLYSIN RELATED TO THE THERMOSTABLE DIRECT HEMOLYSIN OF VIBRIO-PARAHAEMOLYTICUS [J].
NISHIBUCHI, M ;
TANIGUCHI, T ;
MISAWA, T ;
KHAEOMANEEIAM, V ;
HONDA, T ;
MIWATANI, T .
INFECTION AND IMMUNITY, 1989, 57 (09) :2691-2697
[7]   Production of thermostable direct hemolysin by Vibrio parahaemolyticus enhanced by conjugated bile acids [J].
Osawa, R ;
Yamai, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (08) :3023-3025
[8]   Occurrence of urease-positive Vibrio parahaemolyticus in Kanagawa, Japan, with specific reference to presence of thermostable direct hemolysin (TDH) and the TDH-related-hemolysin genes [J].
Osawa, R ;
Okitsu, T ;
Morozumi, H ;
Yamai, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :725-727
[9]   Food-borne disease outbreaks due to bacteria in Taiwan, 1986 to 1995 [J].
Pan, TM ;
Wang, TK ;
Lee, CL ;
Chien, SW ;
Horng, CB .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (05) :1260-1262
[10]  
REED L. J., 1938, AMER JOUR HYG, V27, P493