In order to study low temperature stress effect on wheat cultivars germination, an experiment was carried out in the seed technology laboratory of Mohaghegh Ardabili University in Ardabil, Iran. It was conducted by factorial design based on complete randomized deign (CRD) with two factor and three replications; content 30 grain per replication. Factor A include three temperature levels (2, 3 and 5 degrees C) and factor B, include five wheat cultivars (Gaspard, MV17, Sardary, Cascogen and Bezostaya) were used in this experiment. Result showed that velocity of seed was lowest in 2 degrees C and Gaspard, Sardary, Cascogen and Bezostaya have highest velocity of seed, respectively. Therefore, greatest seed velocities belong to Bezostaya cultivar in 5 degrees C temperature. Lowest seed velocity is related to MV17 in 2 degrees C temperature. For the number of roots, Cascogen cultivar with greatest and Gaspard cultivar with lowest number roots were determined. Sardary cultivar has highest coleoptiles length. In the final result, Bezostaya cultivar was arranged in the first and highest level, between five cultivar for low temperature stress characteristics and Sardary cultivar showed second level, significantly in comparison of the another cultivars.