A flow injection chemiluminescence method for the determination of lincomycin in serum using a diperiodato-cuprate (III)-luminol system

In this paper, the novel trivalent copper-periodate complex {K-5[Cu(HIO6)(2)], DPC} has been applied in a luminol-based chemiluminescence (CL) reaction. Coupled with flow injection (FI) technology, the FI-CL method was proposed for the determination of lincomycin hydrochloride. The CL reaction between luminol and DPC occurred in an alkaline medium. The CL intensity could be greatly enhanced by lincomycin hydrochloride. The relative CL intensity was proportional to the concentration of lincomycin hydrochloride in the range of 1 x 10(-8) to 5 x 10(-6) g mL(-1) and the detection limit was at the 3.5 x 10(-9) g mL(-1) level. The relative standard deviation at 5 x 10(-8) g mL(-1) was 1.7% (n = 9). The sensitive method was successfully applied to the direct determination of lincomycin hydrochloride (ng mL(-1)) in serum. A possible mechanism of the lumonol-DPC CL reaction was discussed by the study of the CL kinetic characteristics and the spectra of CL reaction. The oxidability of DPC was studied by means of its electrochemical response. Copyright (C) 2010 John Wiley & Sons, Ltd.