Grape Seed Proanthocyanidins Exert a Neuroprotective Effect by Regulating Microglial M1/M2 Polarisation in Rats with Spinal Cord Injury

被引：13

作者：

Liu, Wen-zhao ^{[1
,2
]}

Ma, Zhan-jun ^{[1
,3
]}

Kang, Ji-he ^{[1
,2
]}

Lin, Ai-xin ^{[1
,2
]}

Wang, Zhao-heng ^{[1
,2
]}

Chen, Hai-wei ^{[1
,2
]}

Guo, Xu-dong ^{[1
,2
]}

He, Xue-gang ^{[1
,2
]}

Kang, Xue-wen ^{[1
,2
,4
]}

机构：

[1] Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China

[2] Lanzhou Univ, Dept Orthoped, Hosp 2, Lanzhou 730030, Gansu, Peoples R China

[3] UCLouvain, Univ Catholique Louvain, Louvain Drug Res Inst, Adv Drug Delivery & Biomat, B-1200 Brussels, Belgium

[4] Int Cooperat Base Gansu Prov Pain Res Spinal Disor, Lanzhou 730000, Gansu, Peoples R China

来源：

MEDIATORS OF INFLAMMATION | 2022年 / 2022卷

关键词：

COGNITIVE IMPAIRMENT; SIGNALING PATHWAY; PI3K/AKT PATHWAY; BRAIN; INFLAMMATION; ACTIVATION; DISEASE; LIPOPOLYSACCHARIDE; LPS; NEUROTOXICITY;

D O I：

10.1155/2022/2579003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Spinal cord injury (SCI) is a highly disabling disorder for which few effective treatments are available. Grape seed proanthocyanidins (GSPs) are polyphenolic compounds with various biological activities. In our preliminary experiment, GSP promoted functional recovery in rats with SCI, but the mechanism remains unclear. Therefore, we explored the protective effects of GSP on SCI and its possible underlying mechanisms. We found that GSP promoted locomotor recovery, reduced neuronal apoptosis, increased neuronal preservation, and regulated microglial polarisation in vivo. We also performed in vitro studies to verify the effects of GSP on neuronal protection and microglial polarisation and their potential mechanisms. We found that GSP regulated microglial polarisation and inhibited apoptosis in PC12 cells induced by M1-BV2 cells through the Toll-like receptor 4- (TLR4-) mediated nuclear factor kappa B (NF-kappa B) and phosphatidylinositol 3-kinase/serine threonine kinase (PI3K/AKT) signaling pathways. This suggests that GSP regulates microglial polarisation and prevents neuronal apoptosis, possibly by the TLR4-mediated NF-kappa B and PI3K/AKT signaling pathways.

引用

页数：23