Negative regulation of β-catenin/Tcf signaling by naringenin in AGS gastric cancer cell

被引：43

作者：

Lee, JH ^{[1
]}

Park, CH ^{[1
]}

Jung, KC ^{[1
]}

Rhee, HS ^{[1
]}

Yang, CH ^{[1
]}

机构：

[1] Seoul Natl Univ, Div Chem & Mol Engn, Seoul 151742, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 335卷 / 03期

关键词：

beta-catenin/Tcf signaling; naringenin; nuclear beta-catenin; mechanism;

D O I：

10.1016/j.bbrc.2005.07.146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional activation of beta-catenin/Tcf signaling plays an important role in early events in carcinogenesis. We examined the effect of naringenin against beta-catenin/Tcf signaling in gastric cancer cells. Reporter gene assay showed that naringenin inhibited beta-catenin/Tcf signaling efficiently. In addition, the inhibition of beta-catenin/Tcf signaling by naringenin in HEK293 cells transiently transfected with constitutively mutant beta-catenin gene, whose product is not phosphorylated by GSK3 beta, indicates that its inhibitory mechanism was related to beta-catenin itself or downstream components. To investigate the precise inhibitory mechanism, we performed immunofluorescence, Western blot, and EMSA. As a result, our data revealed that the beta-catcnin distribution and the levels of nuclear beta-catenin and Tcf-4 proteins were unchanged after naringenin treatment. Moreover, the binding activities of Tcf complexes to consensus DNA were not affected by naringenin. Taken together, these data suggest that naringenin inhibits beta-catenin/Tcf signaling in gastric cancer with unknown mechanisms. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：771 / 776

页数：6

共 33 条

[1]

Caca K, 1999, CELL GROWTH DIFFER, V10, P369

[2]

Clements WM, 2002, CANCER RES, V62, P3503

[3] The metalloproteinase matrilysin is a target of β-catenin transactivation in intestinal tumors [J].

Crawford, HC ;

Fingleton, BM ;

Rudolph-Owen, LA ;

Goss, KJH ;

Rubinfeld, B ;

Polakis, P ;

Matrisian, LM .

ONCOGENE, 1999, 18 (18) :2883-2891

[4] Tyrosine phosphorylation and cadherin/catenin function [J].

Daniel, JM ;

Reynolds, AB .

BIOESSAYS, 1997, 19 (10) :883-891

[5] Inhibition of β-catenin/Tcf activity by white tea, green tea, and epigallocatechin-3-gallate (EGCG):: minor contribution of H2O2 at physiologically relevant EGCG concentrations [J].

Dashwood, WM ;

Orner, GA ;

Dashwood, RH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (03) :584-588

[6] The nonsteroidal anti-inflammatory drugs aspirin and indomethacin attenuate β-catenin/TCF-4 signaling [J].

Dihlmann, S ;

Siermann, A ;

Doeberitz, MV .

ONCOGENE, 2001, 20 (05) :645-653

[7] MODULATING EFFECT OF PLANT FLAVONOIDS ON THE MUTAGENICITY OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE [J].

FRANCIS, AR ;

SHETTY, TK ;

BHATTACHARYA, RK .

CARCINOGENESIS, 1989, 10 (10) :1953-1955

[8] Frequent β-catenin aberration in human hepatocellular carcinoma [J].

Fujie, H ;

Moriya, K ;

Shintani, Y ;

Tsutsumi, T ;

Takayama, T ;

Makuuchi, M ;

Kimura, S ;

Koike, K .

HEPATOLOGY RESEARCH, 2001, 20 (01) :39-51

[9] Anti-Helicobacter pylori flavonoids from licorice extract [J].

Fukai, T ;

Marumo, A ;

Kaitou, K ;

Kanda, T ;

Terada, S ;

Nomura, T .

LIFE SCIENCES, 2002, 71 (12) :1449-1463

[10] Identification of c-MYC as a target of the APC pathway [J].

He, TC ;

Sparks, AB ;

Rago, C ;

Hermeking, H ;

Zawel, L ;

da Costa, LT ;

Morin, PJ ;

Vogelstein, B ;

Kinzler, KW .

SCIENCE, 1998, 281 (5382) :1509-1512

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