Proteomic Analysis of the Secretory Proteins from Phytophthora infestans under Nitrogen Deficiency using Label-free LC-MS

被引:1
|
作者
Yu, Ping
Dong, Chao
Yao, Chunxin
Ding, Yumei
Zhou, Xiaogang [1 ]
机构
[1] Yunnan Acad Agr Sci, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr, Kunming 650205, Yunnan, Peoples R China
关键词
Effectors; Phytophthora infestans; Proteomics; Secretory proteins; PATHOGEN PHYTOPHTHORA; PLANT-PATHOGENS; EFFECTOR; EXPRESSION; GENE; DISEASE; CLONING; AVR3A;
D O I
10.17957/IJAB/15.0823
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Phytophthora infestans causes the late blight disease in potato, and its effectors have a potential role in its pathogenicity. Nitrogen is a key nutrient source affecting the growth and development of microbiota. Differentially expressed proteins secreted by the P. infestans strain NOD-1 were analyzed using label-free liquid chromatography-mass spectrometry quantitative proteomics in a complete medium or under nitrogen-deficient conditions to understand whether nitrogen stress promoted the secretion of more pathogenic proteins by P. infestans. A total of 5615 unique peptides and 1188 proteins were identified. Moreover, 93 differentially expressed proteins (containing signal peptides of Crinkling and necrosis-inducing protein and Arginine-X-leucine-arginine) and 28 specific proteins (localized extracellularly, in the cytoplasm, and in the plasma membrane) were detected under nitrogen-deficient conditions. Furthermore, 27 important effectors (mainly apoplastic and cytoplasmic), which had a potential role in the infection process, were screened. This study showed that the pathogenicity of P. infestans was enhanced under nitrogen-deficient conditions through the expression of effectors, particularly the cytoplasmic effectors. (C) 2018 Friends Science Publishers
引用
收藏
页码:2363 / 2370
页数:8
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