Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

被引：0
作者：
Cui, J. J. [1 ]
Tian, Y. [1 ]
Liu, Y. [1 ]
Huang, H. L. [1 ]
Jin, Y. [1 ]
机构：
[1] Yanbian Univ, Coll Agr, Yanji, Jinlin, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Boar sperm; Proacrosin; Freeze-thaw; Capacitation; SDS-PAGE; Western blotting; ACROSOME REACTION; CRYOPRESERVATION; RELEASE;
D O I：
10.4238/2014.November.27.20
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group. Fresh spermatozoa displayed 45- and 35-kDa protein bands, while those of freeze-thawed andcapacitated spermatozoa displayed 45-, 35- and 28-kDa bands. In summary, these data indicate that proacrosin is activated, thus becoming alpha-and beta-acrosins and a 28-kDa protein during capacitation and freeze-thawing.
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页码：9915 / 9920
页数：6
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